Batching method of organic substrate in combined anaerobic fermentation and combined anaerobic fermentation method

An organic substrate, anaerobic fermentation technology, applied in the direction of biochemical equipment and methods, fermentation, microbial measurement/inspection, etc., can solve the problems of time-consuming and laborious results, the inability to guarantee the synergistic effect of multiple materials, and lack of universality. Achieve high synergy index, improve synergy effect, and high gas production rate

Pending Publication Date: 2022-07-15
CHINA AGRI UNIV
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Problems solved by technology

Especially with the construction of large-scale and ultra-large bio-natural gas projects, the raw materials for combined anaerobic fermentation tend to be diversified, and it is no longer possible to guarantee and improve the synergistic effect of multiple materials by taking the carbon-nitrogen ratio as the basis for the ingredients of organic substrates for combined anaerobic fermentation
[0004] In order to ensure the fermentation effect of combined anaerobic fermentation with a high synergistic effect index, in practice, a fermentation pre-experiment of a specific substrate to be fermented is usually carried out to determine the appropriate material ratio of the substrate to be fermented, and such a pre-experiment is not only time-consuming and laborious Moreover, the results are not universal. Once the composition of the combined fermentation substrate and its organic component content change, it is necessary to re-perform the fermentation pre-experiment to determine the appropriate ratio again.
There is currently no general and effective ingredient method that can guarantee the co-fermentation of organic substrates without the need for fermentation pre-experiments

Method used

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  • Batching method of organic substrate in combined anaerobic fermentation and combined anaerobic fermentation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Binary distribution ratio:

[0047] Food waste, corn stalk and chicken manure were selected as organic substrate raw materials, and the mass percentage contents of protein, lipid, lignin and volatile solid were detected respectively, and the obtained volatile solid content, protein content, lipid content and volatile solid content were used. The lignin content calculates the carbohydrate content in the organic substrate, said carbohydrate content = volatile solids content - protein content - lipid content - lignin content;

[0048] It is obtained that the mass percentages of carbohydrates, proteins and lipids in the kitchen waste relative to the volatile solids are 75.7%, 18.8% and 0.5%, respectively. The carbohydrates, proteins and lipids of the corn stover are relative to the volatile solids. The mass percentages in the chicken manure were 85.3%, 5.5% and 0, respectively, and the mass percentages of carbohydrates, proteins, and lipids in chicken manure relative to the...

Embodiment 2

[0058] Triple allocation ratio:

[0059] Select corn stalks, chicken manure and kitchen waste as organic substrate raw materials, and detect the mass percentage content of protein, lipid, lignin and volatile solid in the organic substrate respectively, and use the obtained volatile solid content, protein content, lipid content Content and Lignin Content Calculate the carbohydrate content in the organic substrate, the carbohydrate content = volatile solids content - protein content - lipid content - lignin content, resulting in protein content, lipid content and carbohydrate content, respectively content;

[0060] The mass percentages of carbohydrates, proteins, and oils in corn stover relative to volatile solids were 61.61%, 6.48%, and 1.50%, respectively, and the mass percentages of carbohydrates, proteins, and oils in chicken manure relative to volatile solids were The contents are 41.58%, 38.53% and 6.01% respectively, and the mass percentages of carbohydrates, proteins an...

Embodiment 3

[0074] The organic substrate raw materials are corn stover, chicken manure and slaughter waste, and the method for determining the carbohydrate content is the same as that in Example 2. It is obtained that the carbohydrate, protein, and oil content of the corn stover relative to the mass percentage in the volatile solids are respectively: 61.61%, 6.48% and 1.50%, the mass percentages of carbohydrates, proteins and oils in chicken manure relative to volatile solids were 41.58%, 38.53% and 6.01% respectively, and the carbohydrates, proteins and oils in slaughter waste were The mass percentages relative to the volatile solids are 0.10%, 55.73% and 43.91%, respectively;

[0075] The raw material combination contains carbohydrates, proteins and lipids at the same time. According to the mass ratio of corn stover, chicken manure and slaughter waste, the ingredients are 185:936:100 (corn stover is 2.76g, chicken manure is 13.94g, The slaughter waste was 1.49 g), and the mass ratio of ...

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Abstract

The invention belongs to the technical field of combined anaerobic fermentation, and particularly relates to a combined anaerobic fermentation organic substrate proportioning method and a combined anaerobic fermentation method. In the proportioning method provided by the invention, when a substrate meets the conditions that carbohydrate (C) is greater than or equal to 50% VS, protein (P) is greater than or equal to 10% VS and lipid (L) is greater than or equal to 10% VS, organic components for combined anaerobic fermentation are proportioned according to the mass ratio in an area formed by four points, namely 80: 10: 10, 80: 20: 0, 60: 20: 20 and 60: 30: 10, in a C-P-L ternary phase diagram; when C is smaller than 50% VS or P is smaller than 10% VS or L is smaller than 10% VS, the organic substrates are mixed according to the mass ratio of P to L being (3-7): 7 or the mass ratio of L to C being (4-9): 6 or the mass ratio of P to C being 7: (3-28). The batching method provided by the invention has universality, the synergistic effect of the organic matters is remarkably improved, and the high gas yield and the synergistic index are ensured.

Description

technical field [0001] The invention belongs to the technical field of combined anaerobic fermentation, and in particular relates to a method for batching organic substrates in combined anaerobic fermentation and a combined anaerobic fermentation method. Background technique [0002] Anaerobic fermentation refers to the catabolism of organic substrates (such as human, livestock and poultry manure, straw, kitchen waste, etc.) under certain temperature and anaerobic conditions, through the catabolism of various microorganisms, to finally form flammable mixed gases such as methane and carbon dioxide. process. Combined anaerobic fermentation is a technology in which a variety of organic substrates are mixed in a certain proportion and then anaerobic fermentation is carried out. [0003] The ingredients of organic substrates in combined anaerobic fermentation directly determine the pros and cons of the fermentation effect such as gas production (methane production) in combined a...

Claims

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Application Information

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IPC IPC(8): C12P5/02C12Q3/00
CPCC12P5/023C12Q3/00
Inventor 段娜周嘉良陈锐明司齐郑鑫
Owner CHINA AGRI UNIV
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