Unlock instant, AI-driven research and patent intelligence for your innovation.

Detection chip and detection method

A technology for detecting chips and airways, which is applied in chemical instruments and methods, biochemical equipment and methods, and microbial measurement/inspection. It can solve problems such as high cost and complex structure of detection chips, so as to avoid infection and improve accuracy. , the effect of accelerated development

Pending Publication Date: 2022-07-22
SHANGHAI INST OF MICROSYSTEM & INFORMATION TECH CHINESE ACAD OF SCI
View PDF22 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In view of the shortcomings of the prior art described above, the purpose of the present invention is to provide a detection chip and a detection method for solving the problems of complex structure and high cost of the detection chip in the prior art

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Detection chip and detection method
  • Detection chip and detection method
  • Detection chip and detection method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0091] A detection chip is provided in this embodiment, please refer to figure 1 , shown as a schematic diagram of the exploded structure of the detection chip, including an airway layer 1, an elastic membrane layer 2, a fluid layer 3 and a bottom layer 4, wherein the elastic membrane layer 2 is located in the airway layer 1 and the fluid layer 3 In between, the bottom layer 4 is located on the back side of the fluid layer 3 .

[0092] As an example, the airway layer 1 is provided with at least one first clamp positioning hole 104 , the elastic membrane layer 2 is provided with at least one second clamp positioning hole 201 , and the fluid layer 3 is provided with at least one first clamp positioning hole 201 . Three fixture positioning holes 313 , the first fixture positioning hole 104 , the second fixture positioning hole 201 and the third fixture positioning hole 313 are located on a straight line in the vertical direction.

[0093] Specifically, see figure 2 , which is ...

Embodiment 2

[0118] A detection method is provided in the present embodiment, comprising the following steps:

[0119] S1: Step of assembling a PCR tube: Provide the detection chip as described in Embodiment 1, and assemble a PCR tube at the interface of the first PCR tube and the interface of the second PCR tube respectively.

[0120] S2: Lysis step: provide the detection chip as described in Example 1, apply the sample solution and the first reagent in the first PCR tube through the first liquid reservoir and the first PCR tube interface, and pass the external Setting heating equipment to heat the first PCR tube to thermally lyse the sample;

[0121] S3: Mixing step: suction the solution in the first PCR tube through the pipette, the microfluidic channel group and the microvalve assembly to the peripheral component connected to the peripheral component connector, and mix The solution in the peripheral component is injected into one of the second reservoirs to be mixed with a premix (eg ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a detection chip and a detection method. The detection chip comprises an air passage layer, an elastic film layer, a fluid layer and a bottom layer. The detection chip integrates sample pretreatment and PCR nucleic acid amplification functions and can be applied to detection of pathogenic microorganisms and the like, the chip controls opening and closing of a micro valve in the chip by means of external air pressure, a piston drives liquid in a micro channel to flow, and a PCR tube connected with the chip can be heated by means of external heating equipment. The chip can realize all functions required by pathogenic microorganism detection, including cell lysis, mixing of a lysis solution and PCRmix, average distribution of a mixed solution into a plurality of PCR tubes connected with the chip, PCR amplification, and real-time recording of a PCR process by a fluorescence detector. The integrated and automatic operation process is convenient to use, infection of operators is avoided, and the result accuracy is improved. The method is expected to accelerate the development of a pathogenic microorganism detection technology and is widely used in hospitals.

Description

technical field [0001] The invention belongs to the field of biological detection, and relates to a detection chip and a detection method. Background technique [0002] Infectious diseases caused by pathogenic microorganisms are a major killer of human beings. Pathogenic microorganisms include viruses, mycoplasmas, bacteria, fungi and parasites. At present, the main difficulty in the treatment of infectious diseases lies in the diagnosis of pathogenic microorganisms, and there is an urgent need for a fast and accurate method to detect and analyze the information of pathogenic microorganisms. [0003] The traditional culture method is the "gold standard" for the detection of pathogenic microorganisms. Specifically, human sputum, blood, etc. are cultured in a specific medium, so that the pathogenic microorganisms can grow into a state visible to the naked eye, and the type of pathogenic microorganisms can be judged by experience. The disadvantage of this method is mainly tha...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): B01L3/00C12M1/34C12M1/38C12M1/02C12M1/00C12Q1/686
CPCB01L3/50273C12Q1/686C12Q2565/629C12Q2563/107
Inventor 吴蕾周洪波贾春平武振华赵辉赵建龙
Owner SHANGHAI INST OF MICROSYSTEM & INFORMATION TECH CHINESE ACAD OF SCI
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com