Culture medium for microbial culture and microbial culture method

Abstract

The invention discloses a culture medium for microbial culture and a microbial culture method, belongs to the technical field of microbial culture, and aims to solve the problems that the existing culture medium is sterilized after preparation, but the sterilization quality is low, and the sterilization time is long, so that on one hand, the working efficiency is influenced; on the other hand, protein in the culture medium can be damaged due to long-time high temperature and high pressure. The method comprises the following steps: putting a culture dish into a high-pressure steam sterilization box, adjusting the temperature to 128 DEG C, sterilizing for 1.5 min, paying attention to the color in the culture dish all the time, completing sterilization, inoculating nutrient solutions with various concentrations, lightly shaking up the inoculated culture dish, storing the culture dish in a constant-temperature box, controlling the temperature of the constant-temperature box to be 37.5 DEG C, and completing culture. The prepared culture medium is good in sterilization effect, protein components in the culture medium are less damaged, and the practicability is high.

Description

technical field [0001] The invention relates to the technical field of microorganism culture, in particular to a culture medium for microorganisms and a microorganism culture method. Background technique [0002] Microorganisms include: bacteria, viruses, fungi, and some small protists, microscopic algae, etc., a large group of biological groups, which are small and closely related to humans. Covering many types of beneficial and harmful, it is widely involved in many fields such as food, medicine, industry and agriculture, environmental protection, sports and so on. In Chinese textbooks, microorganisms are divided into the following eight categories: bacteria, viruses, fungi, actinomycetes, rickettsia, mycoplasma, chlamydia, and spirochetes. Some microorganisms are visible to the naked eye, such as mushrooms belonging to fungi, Ganoderma lucidum, shiitake mushrooms, etc. [0003] After the current medium is prepared, the medium will be sterilized, but the quality of the s...

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Problems solved by technology

[0005] The purpose of the present invention is to provide a culture medium for microorganisms and a method for culturing microorganisms, which solves the problem that the current culture medium in the background technology will sterilize the culture medium after the preparation is completed, but the quality of the sterilization is low and the sterilization time is relatively long. On the one hand, it affects work efficiency, on the other hand, long-term high temperature and high pressure will cause damage to the protein in the medium. Secondly, in the process of cultivating microorganisms, especially after adjusting the pH, the petri dish needs to be sterilized. The current sterilization effect is also biased, leading to the problem of low quality of petri dishes

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