Integrated polymerase chain reaction detection device

Abstract

The invention discloses an integrated polymerase chain reaction detection device, which comprises a flat plate reactor, a sample introduction rod and a detection rod, the flat plate reactor comprises a flat plate body and a sealing film, the flat plate body is provided with a reaction tank, the reaction tank is respectively provided with a sample introduction hole and a sample discharge hole, the sealing film seals the reaction tank to form a reaction cavity, and the detection rod is arranged in the reaction cavity. The sample introduction rod comprises a sample introduction rod body, one end of the sample introduction rod body is provided with a sample bin, the detection rod comprises a detection rod body, the detection rod body is provided with a test paper groove, the sample introduction rod body and the detection rod body are rotationally connected with the flat plate body, and when the sample introduction rod body rotates to the communication position, the sample bin is communicated with the sample introduction hole; when the sample injection rod body rotates to the isolation position, the sample bin is not communicated with the sample injection hole, when the detection rod body rotates to the communication position, the test paper groove is communicated with the sample outlet hole, and when the detection rod body rotates to the isolation position, the test paper groove is not communicated with the sample outlet hole. The device is simple in structure and high in integration degree, and polymerase chain reaction and detection are integrated together.

Description

technical field [0001] The invention relates to the technical field of polymerase chain reaction devices, in particular to an integrated polymerase chain reaction detection device. Background technique [0002] The full name of polymerase chain reaction detection technology is fluorescence quantitative polymerase chain reaction. As the most common method for detecting virus-specific sequences, it was first proposed in the 1970s. Finally, the polymerase chain reaction was invented by an American company, using heat-resistant DNA. Polymerases mimic the process of DNA replication in vivo, and it is now possible to amplify a specific segment of DNA in vitro. The specific process of polymerase chain reaction is as follows: first, high temperature denaturation and heating template DNA double-strand hydrogen bonds are broken to obtain two single strands, low temperature annealing allows the suddenly cooled template DNA to be complementary to the primer according to the base pairing...

AI Technical Summary

Problems solved by technology

[0004] At present, in China, most of the polymerase chain reaction instruments are invented and designed, and most of the consumables for polymerase chain reaction detection are centrifuge tubes, which require manual operation. Each centrifuge tube is independent of each other, and the operation is cumbersome; The optimization of the integration direction is mostly designed for sample liquid transfer and mixing, and nesting is based on the centrifuge tube shape, but this type of method has the following problems: 1. Lack of structural flexibility, and it is inconvenient to add new substances or new processes; 2. 1. There is still human intervention in some steps such as detection and sampling; 3. There is a possibility of contamination if the non-full process is closed; 4. The independence of each reaction area may be biased, and there may be cross-effects. High biological purity requirements require sufficient sensitivity to withstand changing temperatures, and the introduction of impurities is something that is not allowed in most experiments. Of course, the independence and integrity of the entire reaction equipment are highly required

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