Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

SNP (Single Nucleotide Polymorphism) site for detecting mengbo-like variant

A site and blood type technology, applied in the direction of recombinant DNA technology, microbial measurement/testing, DNA/RNA fragments, etc., can solve problems such as hemolytic transfusion reactions, and achieve rapid detection results

Pending Publication Date: 2022-08-02
QINGDAO BLOOD CENT
View PDF0 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Due to the lack of H antigen on the red blood cells of Bombay-like individuals and the presence of anti-HI antibodies in the serum, routine ABO blood group testing is easy to mistake the Bombay-like blood type for O type, and if O-type red blood cells are transfused, hemolytic transfusion reactions will occur

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • SNP (Single Nucleotide Polymorphism) site for detecting mengbo-like variant
  • SNP (Single Nucleotide Polymorphism) site for detecting mengbo-like variant
  • SNP (Single Nucleotide Polymorphism) site for detecting mengbo-like variant

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1: Screening of gene polymorphism site markers

[0027] 1. Extract peripheral blood genomic DNA:

[0028]Under the premise of complying with the relevant national policies, and on the basis of obtaining the informed consent of the sampling subjects, collect 5 mL of peripheral venous blood from patients with suspected Bombay blood type, put it in an EDTA anticoagulation tube, take 500 μL into a centrifuge tube, add an equal volume of Red blood cell lysate (pH 8.0) was vortexed for 5 minutes, centrifuged at 12,000 rpm for 5 minutes, and the supernatant was discarded. Repeatedly adding 500ul of red blood cell lysate, vortexing for 5 minutes, centrifuging at 12000rpm for 5 minutes, and discarding the supernatant. The precipitate was vortexed for 5 minutes, 50 ul of nuclear lysis buffer was added, vortexed for 5 minutes, and placed in a metal bath at 56°C for 15 minutes. Remove the sample from the water bath and add 135ul of protein removal solution. Vortex to mix...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides an SNP (Single Nucleotide Polymorphism) site for detecting a mengbloody-like variant. The provided SNP site is positioned in a fourth exon coding region sequence of a human FUT1 gene of which the nucleotide sequence is SEQ ID NO: 1; a 35th basic group nt35C is greater than T; the base nt422G at the 422nd site is greater than T; and the basic group nt422G at the 803rd site is greater than T. The invention also provides an application of the preparation for detecting the SNP site in preparation of a product for detecting the mengbuy-like blood type. The gene polymorphism site is obtained by detecting and screening, an effective way for diagnosing the Bengal-like blood type gene is provided, and the application effect shows that the gene polymorphism site and the detection primer provided by the invention can be effectively used for rapidly detecting the Bengal-like blood type gene for blood donors and clinical patients.

Description

technical field [0001] The invention belongs to the technical field of genetic diagnosis products, and in particular relates to a SNP site for detecting Bombay-like blood group variant type, a molecular preparation for detecting the SNP site and its application. [0002] Background of the Invention [0003] The blood group system refers to a classification system established by classifying blood based on differences in antigens on the red blood cell membrane. As of February 2021, a total of 43 human red blood cell blood group systems have been confirmed by the International Society for Blood Transfusion (ISBT). Among them, H antigen belongs to the Hh blood group system, which is closely related to the ABO blood group and Lewis blood group system, and is located on the oligosaccharide branched molecular structure outside the red blood cell membrane. [0004] The Hh blood group system mainly includes two phenotypes, Bombay type and Bombay-like type, which are characterized by ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883C12N15/11
CPCC12Q1/6883C12Q2600/156
Inventor 韩斌侯云迟晓云冯智慧
Owner QINGDAO BLOOD CENT
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com