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Preparation method of reticulocyte quality control substance based on fluorescence principle

A technology of erythrocytes and erythrocytes, which is applied in the field of preparation of reticulocyte quality control substances based on the principle of fluorescence, and can solve the problems of increasing production costs

Pending Publication Date: 2022-08-02
URIT MEDICAL ELECTRONICS CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a method for preparing reticulocyte quality control substances based on the principle of fluorescence, aiming to solve the problem that the cells need to be stained with protein fluorescent dyes when preparing reticulocytes, which increases the production cost

Method used

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  • Preparation method of reticulocyte quality control substance based on fluorescence principle
  • Preparation method of reticulocyte quality control substance based on fluorescence principle
  • Preparation method of reticulocyte quality control substance based on fluorescence principle

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preparation example Construction

[0041] see Figure 1 to Figure 7 The present invention provides a method for preparing a reticulocyte quality control substance based on a fluorescence principle, comprising the following steps:

[0042] S1 washes and separates anticoagulated blood to obtain anucleated red blood cells;

[0043] The specific method is as follows: S11 mixes the anticoagulant with blood to obtain anticoagulated blood;

[0044] Specifically, the anticoagulant includes an EDTA anticoagulant or a citric acid anticoagulant;

[0045] S12 washes and separates the anticoagulated blood to obtain anucleated red blood cells.

[0046] S2 uses a cell solidifying agent to process and fix the anucleated red blood cells to obtain solidified red blood cells;

[0047] Specifically, the dosage of the cell fixative includes 5-20 times the volume of the anucleated red blood cells, and the greater the dosage of the cell fixative, the faster the reaction process.

[0048] The cell fixative includes aldehyde cell f...

Embodiment 1

[0068] Fresh anticoagulated pig blood was collected using anticoagulant. Pig blood was centrifuged at 2000 rpm for 10 minutes. The plasma, platelet layer and leukocyte layer are removed after centrifugation, leaving purified red blood cells. Add 10 times the volume of erythrocyte volume to the erythrocytes, and place it at room temperature for 40 minutes after mixing, and mix it every 10 minutes. After the treatment, centrifuge at 2000 rpm for 5 minutes, pour off the supernatant, add cell maintenance solution 3 times the volume of the remaining cells, add 5% of the total volume of the de-solidifying agent solution after mixing, and act at room temperature for 30 minutes. Repeat washing the cells two to three times with the cell maintenance solution, and then transfer the cells to an appropriate amount of cell maintenance solution for preservation, which can be used as a reticulocyte simulant. Red blood cell mimics, white blood cell mimics, platelet mimics, and nucleated red ...

Embodiment 2

[0070] Fresh anticoagulated pig blood was collected using anticoagulant. Pig blood was centrifuged at 2000 rpm for 10 minutes. The plasma, platelet layer and leukocyte layer are removed after centrifugation, leaving purified red blood cells. Add 10 times the volume of erythrocytes to the erythrocytes, and place the cell solidifying agent 2 at room temperature for 30 minutes after mixing, and mix once every 10 minutes. After the treatment, centrifuge at 2000 rpm for 5 minutes, pour off the supernatant, add cell maintenance solution 3 times the volume of the remaining cells, add 5% of the total volume of the de-solidifying agent solution after mixing, and act at room temperature for 30 minutes. Repeat washing the cells two to three times with the cell maintenance solution, and then transfer the cells to an appropriate amount of cell maintenance solution for preservation, which can be used as a reticulocyte simulant. Red blood cell mimics, white blood cell mimics, platelet mimi...

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Abstract

The invention relates to the technical field of blood detection quality control substances, in particular to a reticulocyte quality control substance preparation method based on a fluorescence principle, which comprises the following steps: washing and separating anticoagulant blood to obtain seedless red blood cells; treating and fixing the seedless red blood cells by using a cell curing agent to obtain cured red blood cells; performing curing agent removal treatment on the cured red blood cells to obtain red blood cell products; the erythrocyte product is washed and then stored in a cell maintenance solution, a reticulocyte simulant is obtained, the reticulocyte quality control material is prepared by combining the prepared reticulocyte simulant with erythrocytes, and the problems that when the reticulocytes are prepared, a protein fluorescent dye needs to be used for dyeing the cells, and the dyeing time is short are solved. And the manufacturing cost is increased.

Description

technical field [0001] The invention relates to the technical field of blood detection quality control substances, in particular to a method for preparing a reticulocyte quality control substance based on a fluorescence principle. Background technique [0002] Reticulocytes are immature erythrocytes that are intermediate between late immature erythrocytes and mature erythrocytes. It is an important indicator to reflect the hematopoietic function of the erythroid of the bone marrow and to judge the curative effect of anemia and related diseases. Quality controls are used to check the performance of analytical instruments or methods and can ensure the reliability of laboratory results. The nature of the control samples should be as close as possible to the actual clinical samples tested, while at the same time ensuring suitability for the test system being monitored. [0003] At present, the prior art (CN112986589A) discloses a method for preparing reticulocyte-simulating pa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28G01N1/34G01N1/40G01N21/64G01N33/96
CPCG01N1/28G01N1/34G01N1/4077G01N33/96G01N21/6486G01N2001/4083
Inventor 龚喜周永杨祝毅
Owner URIT MEDICAL ELECTRONICS CO LTD
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