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Anti-mutant citrullinated waveform protein antibody detection kit and application thereof

A citrullinated and vimentin technology, applied in the field of anti-mutant citrullinated vimentin antibody detection kits, can solve problems such as low sensitivity and specificity, and achieve the effects of avoiding deviation of results, fast detection, and improving work efficiency

Pending Publication Date: 2022-08-02
ZHUHAI LIVZON DIAGNOSTICS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The object of the present invention is to provide an anti-mutant citrullinated vimentin antibody detection kit and its application to solve the problem of low sensitivity and specificity of anti-mutant citrullinated vimentin antibody detection reagents in the prior art

Method used

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  • Anti-mutant citrullinated waveform protein antibody detection kit and application thereof
  • Anti-mutant citrullinated waveform protein antibody detection kit and application thereof
  • Anti-mutant citrullinated waveform protein antibody detection kit and application thereof

Examples

Experimental program
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Effect test

experiment example 11

[0057] The mutant citrullinated vimentin antigen used in this example is mutant citrullinated vimentin polypeptide E1. The amino acid sequence of E1 is SEQ ID No. 1, wherein X in the amino acid sequence is citrulline Cit. The mutant citrullinated vimentin polypeptide E1 is modified with biotin.

[0058] The magnetic particles used in this example are magnetic barcode particles coupled with streptavidin. A magnetic barcode particle has multiple barcode regions. The magnetic barcode particles in the streptavidin-coupled magnetic barcode particle suspension are magnetically separated to obtain the magnetic barcode particles. The magnetic barcode microparticles were resuspended to a concentration of 2 × 10 in phosphate buffer with a pH of 7.0 and a concentration of 0.05 moL / L. 5 particles / mL of magnetic barcode microparticle solution. Wash three times with pH 7.0 and 0.05moL / L phosphate buffer, and resuspend to 2 × 10 5 particles / mL of magnetic barcode microparticle solution....

experiment example 12

[0073] The mutant citrullinated vimentin antigen is mutant citrullinated vimentin polypeptide H1. The amino acid sequence of H1 is SEQ ID No. 2, wherein X in the amino acid sequence is citrulline Cit. The rest of the conditions were the same as in Experimental Example 1, and the test results of anti-mutated citrullinated vimentin antibodies are shown in Table 2.

experiment example 13

[0075] The mutant citrullinated vimentin antigen is mutant citrullinated vimentin polypeptide G5. The amino acid sequence of H1 is SEQ ID No. 3, wherein X in the amino acid sequence is citrulline Cit. The rest of the conditions were the same as in Experimental Example 1, and the test results of anti-mutant citrullinated vimentin antibodies are shown in Table 3.

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Abstract

The invention provides an anti-mutant citrullinated waveform protein antibody detection kit and application thereof, and relates to the technical field of biology. The kit comprises a mutant citrullinated waveform protein coupled magnetic particle working solution, wherein the working solution is prepared by mixing a buffer solution and mutant citrullinated waveform protein polypeptide E1 coupled magnetic particles, 0.3 ng / (* mL) mutant citrullinated waveform protein polypeptide H1 coupled magnetic particles and 0.3 ng / (* mL) mutant citrullinated waveform protein polypeptide G5 coupled magnetic particles according to a final feed ratio; the tracer-labeled antibody working solution is composed of a tracer-labeled anti-human IgM antibody and a diluent. According to the kit disclosed by the invention, the mutant citrullinated waveform protein polypeptide E1 coupled magnetic particles, the mutant citrullinated waveform protein polypeptide H1 coupled magnetic particles and the mutant citrullinated waveform protein polypeptide G5 coupled magnetic particles are mixed, so that the sensitivity and the specificity are greatly improved.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to an anti-mutant citrullinated vimentin antibody detection kit and application thereof. Background technique [0002] The glycine residue in native vimentin was mutated to arginine, and after citrullination of arginine by peptidyl arginine imidase, the potential protein determinant of citrulline was added, and the structure of vimentin was changed. , resulting in mutant citrullinated vimentin. [0003] Compared with the anti-cyclic citrullinated peptide antibody, the anti-mutated citrullinated vimentin antibody has a higher value in the auxiliary diagnosis of rheumatoid arthritis (RA), especially in the early stage of rheumatoid arthritis. sensitivity and specificity. Anti-mutated citrullinated vimentin antibodies appear earlier than other markers in the serum of patients with rheumatoid arthritis. By detecting changes in the levels of anti-mutated citrullinated vimentin antibodies, ...

Claims

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Application Information

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IPC IPC(8): G01N33/532G01N33/536G01N33/543G01N33/68
CPCG01N33/532G01N33/536G01N33/54326G01N33/68
Inventor 胡卫国孙超张晨光胡林
Owner ZHUHAI LIVZON DIAGNOSTICS
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