A kit of carbamyl ornithine fibre connexin consists of pre-coated carrier of antifebrin connexin antibody (ACA), labeled anticarbamyl ornithine antibody (LAOA), diluting liquid, washing solution, color developing agent and terminating liquid. The detecting method includes adding blood plasma in each hole of plate pre-coated with ACA, adding LAOA and adding color developing agent in sequence for reacting, using enzyme mark scale, counter and detector for detecting, comparing detected from sample hole to detected data from control hole and then calculating for result.