11 results about "Citrullination" patented technology

The invention provides antibodies or binding fragments thereof directed against citrulline-containing epitopes. The antibodies or binding fragments thereof of the invention can be used in therapy, for example in the treatment or prevention of Neutrophil Extracellular Trap (NET)-associated pathologies.

The application discloses citrullinated collagen type II polypeptide and application thereof. The amino acid sequence of the polypeptide is Ste-QYMCitADQAAGGLR (SEQ ID NO. 1), wherein the N terminal of glutamine Q in the sequence is connected with octadecanoic acid Ste, and the fourth position in the sequence is modified by citrullination. The citrullinated collagen type II polypeptide has a long drug half-life, can effectively treat rheumatoid arthritis, and has small side effects.

The application discloses application of citrullinated galectin-1 polypeptide in preparation of a rheumatoid arthritis diagnosis product, and belongs to the technical field of rheumatoid arthritis diagnosis. The amino acid sequence of the citrullinated galectin-1 polypeptide is GD-Cit-WSSQQGSKAVYPE; the product is used for detecting the content of anti-citrullinated galectin-1 polypeptide antibody in a biological sample of a patient, and the antibody level is significantly different between RA patients, normal healthy people and other common rheumatic immune disease patients who are easily confused with rheumatoid arthritis. The product has good sensitivity to common RA, RA with normal ESR and CRP, and anti-CCP antibody / RF negative RA patients, and has good supplementary diagnosis value for the diagnosis of RA.

To provide antibodies or binding fragments thereof directed against citrulline-containing epitopes.SOLUTION: An antibody or binding fragment thereof that specifically binds to a citrullinated epitope on deiminated human histone 2A and / or histone 4 comprises CDR having a particular amino acid sequence. The antibodies or binding fragments thereof of the present invention can be used in therapy, for example, in the treatment or prevention of Neutrophil Extracellular Trap (NET)-associated pathologies.SELECTED DRAWING: None

Disclosed herein are chimeric antigen receptors (CARs) comprising an antigen binding site that recognizes citrullinated polypeptides. Citrullinated polypeptides, such as citrullinated vimentin, are expressed in the skin lesions of subjects with hidradenitis suppurativa. Further disclosed are T cells, and in particular, Treg cells that express these CARs. Administration of these CAR-T cells is useful in the treatment of hidradenitis suppurativa.

The application discloses citrullinated T200C polypeptide and application thereof in preparation of a rheumatoid arthritis diagnosis product, and belongs to the technical field of disease diagnosis. The amino acid sequence of the citrullinated T200C polypeptide is as follows: GSM-Cit-GA-Cit-Cit-EPEEPEGAV. Research finds that the level of anti-citrullinated T200C polypeptide antibodies in the body of RA patients is obviously higher than that of healthy people and other rheumatic immune disease patients who are easily confused with RA. Taking the citrullinated T200C polypeptide of the application as a detection antigen, detection of anti-citrullinated T200C polypeptide antibodies in the body of patients can effectively identify RA, especially for RA with negative anti-CCP antibodies and / or RF, and normal ESR and / or CRP RA, and has good diagnostic value.

The invention discloses a lignin carbon fiber-based microneedle electrode which comprises a working microneedle electrode, a counter microneedle electrode and a reference microneedle electrode, the working microneedle electrode is a specific biological recognition interface constructed by depositing gold on a microneedle substrate, bridging through a sulfydryl-polyethylene glycol-carboxyl self-assembled molecular layer and covalently coupling an H3Cit antibody by utilizing an NHS / EDC.HCl activation method; the microneedle substrate is prepared by ball-milling lignin carbon fibers, ultrasonically dispersing the lignin carbon fibers in a dichloromethane solution of a polylactic acid-glycolic acid copolymer to obtain slurry, injecting the slurry into a microneedle array mold, and performing vacuum defoaming. The invention discloses a three-electrode sensor which comprises a working electrode, a reference electrode and a counter electrode, and a working microneedle electrode is used as the working electrode. The invention discloses an application of a three-electrode sensor in detection of H3Cit. The lignin carbon fiber-based microneedle electrode disclosed by the invention is regular in arrangement, good in mechanical property and capable of effectively identifying H3Cit.

Described herein are methods and kits for diagnosing or determining a progression of rheumatoid arthritis (RA), comprising detecting or determining levels of autoantibodies specific to citrullinated-CLEC12A (C- type lectin domain family 12 member A).

The disclosure relates to novel citrullinated polypeptides that bind to anti-citrullinated protein antibodies (ACPAs) and their use in the diagnosis and prediction of rheumatoid arthritis.