Zipper fastener structure for promoting formation of protein dimers and application thereof

Abstract

The invention belongs to the field of gene engineering, and relates to a zipper fastener structure for promoting formation of protein dimers and an application thereof, and the zipper fastener can beused for dimerization of homologous proteins and dimerization of heterologous proteins, and can also be used for polypeptide cyclization, dimerization of polypeptides and extension of polypeptides. Some examples can obtain an ESAT6-CFP10 dimer close to native conformation, wherein the dimer has better solubility and has a better stimulation effect on memory T cells than ESAT6-CFP10 protein expressed by linear fusion. The inventor also finds that the dimer zipper fastener can help to form more stable cyclic polypeptide, and the CCP polypeptide added with the dimer fastener can increase the detection rate of citrullinated antibody in serum of rheumatoid arthritis patients.

Description

technical field [0001] The invention relates to the field of biology, in particular to the field of proteins, in particular to a zipper button structure for promoting the formation of protein dimers and its application. Background technique [0002] A protein dimer is a protein quaternary structure. Homodimers are formed by combining two identical protein molecules (a process called homodimerization), while heterodimers are formed by two different proteins (called heterodimerization). dimerization). Most dimers in biochemistry are not linked by covalent bonds. For example, reverse transcriptase is a non-covalently linked heterodimeric enzyme linked by two different amino acid chains. Another exception is the dimeric protein NEMO, a dimer linked by disulfide bonds. Some proteins contain specific regions that ensure the formation of dimerization (dimerization domains). In cell growth, reproduction, and signal transduction, protein dimerization plays an important role in t...

AI Technical Summary

Problems solved by technology

The fusion protein portion of this structure is too large to be suitable as a dimeric fusion protein

Disulfide bonds are often used in the dimer design of proteins, but the problems they pose often outweigh the benefits

[0012] CN102191209A discloses a VEGF165 and Ang-1 dual-gene co-expression vector and its application. In the dual-gene co-expression vector pAdTrack-CMV-Ang-1-IRES-VEFG165, human genes VEGF165 and Ang-1 enter through internal ribosomes The internal ribosome entry site (IRES) connection improves the expression level of VEGF165 to a certain extent. However, in the process of vector construction, the VEGF165 and Ang-1 genes are still transferred to the vector plasmid in two steps, and the process is cumbersome and inefficient

However, with the combined diagnosis of CCP, RF, GPI, and AKA currently used clinically, there are still some RA patients (especially early RA patients) who cannot be detected in time.

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