Myristicylation IP enrichment click chemistry kit and use method thereof

Abstract

The invention discloses a myristicylation IP enrichment click chemistry kit and a use method thereof, and relates to the technical field of protein detection. The kit comprises a first solution, a second solution, a third solution, a fourth solution, a fifth solution and a 50 mM tris (2-carboxyethyl), wherein the first solution is prepared from tetradecanoic acid and dimethyl sulfoxide which are subpackaged and cryopreserved at-20 DEG C; the second solution adopts a lysis solution; the third solution adopts a labeling buffer solution, the fourth solution adopts 10 mM of azido diazo biotin, the fifth solution is prepared from 50 mM of copper sulfate and 0.08-10 ml of water, and the third solution adopts 50 mM of tris (2-carboxyethyl); the sixth solution is composed of phosphine and 2.8665 g-10 ml of water, and the seventh solution is confining liquid. Compared with a common method, the myristoyl IP enrichment click chemical kit has the advantages that more peptide fragments can be enriched, more myristoyl modified proteins can be analyzed, the detail degree and the effect which are better than those of the conventional method can be obtained, and the sensitivity is improved.

Description

technical field [0001] The invention belongs to the technical field of protein detection, in particular to a myristoylation IP enrichment click chemistry kit and a method for using the myristoylation IP enrichment click chemistry kit. Background technique [0002] Myristoylation is a type of post-translational modification of proteins with larger molecular weight. Under the catalysis of NMT, the glycine at the N-terminal of the protein reacts irreversibly with myristic acid to form a bond. NMTs bind to a specific set of target protein N-terminal amino acid residues, where residues have been identified as a typical motif, although some amino acid variations may prevent or facilitate this modification in other parts. Another substrate for NMT is myristoyl-CoA thioester, which is produced by intracellular acyl-CoA synthase. In eukaryotes, myristate is introduced by co-translation, when proteolytic digestion produces a new N-terminal glycine, lipidation facilitates protein-prot...

AI Technical Summary

Problems solved by technology

Then for some proteins whose N-terminus participates in some downstream biological functions, this method is also incompatible

Different labeling probes in the existing related technologies can only be used for one detection method. The disadvantage is that the compatibility is insufficient. The problem that can be solved is to adapt to different methods for detection.

Images