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Method for separating and purifying ganoderan based on field flow separation technology

A technology for the separation and purification of Ganoderma lucidum polysaccharides, which is applied in the chemical industry, general water supply conservation, ultrafiltration, etc., and can solve problems such as low efficiency, affecting the biological activity of polysaccharides, and breaking polysaccharide glycosidic bonds.

Pending Publication Date: 2022-08-09
HEBEI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the Sevag method and trichloroacetic acid method commonly used to remove protein from polysaccharides are inefficient and require repeated removal of protein (often more than ten times), which can easily cause polysaccharide glycosidic bonds to break and affect the biological activity of polysaccharides. At the same time, a large amount of organic solvents are used

Method used

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  • Method for separating and purifying ganoderan based on field flow separation technology
  • Method for separating and purifying ganoderan based on field flow separation technology
  • Method for separating and purifying ganoderan based on field flow separation technology

Examples

Experimental program
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Effect test

Embodiment 1

[0058] The crude polysaccharide solution of Ganoderma lucidum was subjected to protein removal and ultrafiltration according to the method of Comparative Example 9. The ultrafiltration time was 45 minutes. The AF4 separation conditions were: the injection volume was 100 μL, the detector flow rate was 1.0 mL / min, and the initial cross-flow flow rate was 2.6 mL. / min exponentially decays to 0.05mL / min with a half-life of 0.8min.

Embodiment 2

[0060] The crude polysaccharide solution of Ganoderma lucidum was subjected to protein removal and ultrafiltration according to the method of Comparative Example 9. The ultrafiltration time was 45 minutes. The AF4 separation conditions were: the injection volume was 100 μL, the detector flow rate was 1.0 mL / min, and the initial cross-flow flow rate was 3.0 mL. / min exponentially decays to 0.05mL / min with a half-life of 0.8min.

[0061] The detection results obtained from Examples 1 to 2 and Comparative Examples 11 to 13 are as follows Figure 5 and 6 shown. Depend on Figure 5 It can be seen that after protein removal for 4 times, the carrier liquid is ultrapure water, the ultrafiltration membrane is a 10kDa renewable cellulose (RC) membrane, the ultrafiltration flow rate is 0.2mL / min, and the ultrafiltration time is 45min, and the flow rate increases with the cross flow. , the separation degree of polysaccharide components gradually became better, and when the initial cros...

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Abstract

The invention provides a method for separating and purifying ganoderan based on a field flow separation technology. According to the method, extracted ganoderma lucidum crude polysaccharide is firstly subjected to protein removal for four times by adopting a Sevag method, then passes through an AF4 pool channel, is subjected to ultrafiltration for a period of time by adopting ultrapure water as a carrier liquid and adopting a 10kDa renewable cellulose membrane at an ultrafiltration flow rate of 0.2 mL / min, and finally is subjected to asymmetric field flow separation, ultrapure water is adopted as a carrier liquid during asymmetric field flow separation, and ultra-pure water is adopted as an ultra-pure liquid during asymmetric field flow separation. And the initial cross flow velocity index is reduced to 0.05 mL / min. According to the method, the protein removal frequency is reduced in the ganoderma lucidum polysaccharide separation and purification process, the carrier liquid in the whole process is ultrapure water, the dialysis desalting step is not needed, the separation and purification efficiency is improved on the basis of protecting the activity of the ganoderma lucidum polysaccharide, and the high-purity ganoderma lucidum polysaccharide is obtained.

Description

technical field [0001] The invention relates to the technical field of separation and purification of Ganoderma lucidum polysaccharide, in particular to a method for separating and purifying Ganoderma lucidum polysaccharide based on field flow separation technology. Background technique [0002] Ganoderma lucidum (Ganoderma lucidum) is the dried fruiting body of Ganoderma lucidum or purple lucidum, a Polyporaceae fungus. In my country, Ganoderma lucidum has a medicinal history of more than 2,000 years. According to "Shen Nong's Materia Medica", Ganoderma lucidum has the effect of strengthening the body, nourishing and strengthening, and prolonging life. Polysaccharide is one of the main active components of Ganoderma lucidum, so the preparation of high-purity Ganoderma lucidum polysaccharide is a necessary condition to study its structure-activity relationship. [0003] The separation and purification of natural plant polysaccharides is a time-consuming and cumbersome proc...

Claims

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Application Information

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IPC IPC(8): C08B37/00B01D61/14
CPCC08B37/0003Y02P20/10
Inventor 窦海洋郭雨曦孙潇李月秋
Owner HEBEI UNIVERSITY