Method for preparing an N-[(aliphatic or aromatic) carbonyl]-2-aminoacetamide compound and a cyclyzed compound
A kind of compound, the technology of alkylamino, which is applied in the field of preparing N-[carbonyl]-2-aminoacetamide compound and its cyclization compound
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Embodiment 1
[0537] Solutions of compounds of formula (I) by the '3-step-one-pot' method using the Ugi multi-component reaction Composite
[0538] Using an equivalent (0.1 ml) 0.1 M solution of the four appropriate components of the compounds of formula (XIV), (XV), (XVI) and (IXb), due to 100% conversion, 10 μmol of the final product is theoretically generated 1,4 - Benzodiazepine-2,5-dione (I). This 4-component condensation was carried out in methanol at room temperature and the solvent was evaporated at 65 °C (using SAVANT _ evaporator for 2 hours). The deprotection / cyclization step was performed with acetyl chloride in 10% methanol or trifluoroacetic acid in 10% dichloroethane. The solvent is then evaporated at 65°C to give the cyclization product compound of formula (I). Use 10%-100% H with 0.1% TFA 2 O / CH 3 C18 Hypersil BDS 3m 4.6×50mm column (UV220nm) with CN mobile phase, 15min Lc / ms analysis (liquid chromatography / mass spectrometry) at a speed of 1ml / min. The desired pro...
Embodiment 2
[0552] General Solid-Phase Synthesis of Compounds of Formula (I) Using Ugi Reaction and Resin (IXa)
[0553] 60 mg of resin (IXa) were preswelled with THF. 0.5M THF:MeOH (1:1) solution containing aldehyde (XV) (10 equivalents), amine (XVI) (10 equivalents) and carboxylic acid (XIV) (10 equivalents) was sequentially added to resin (IXa), The reaction was stirred at room temperature for 3 days. use CH 2 Cl 2 , THF, DMF, THF and MeOH were washed sequentially and the resin was dried in high vacuum to obtain the resin-bound Ugi product (IXa). use BOC 2 O (10 equivalents), Et 3 N (10 equiv) and CH of DMAP 2 Cl 2 Solution work-up (15 hours) gave the active resin (XX) for cleavage. A THF:MeOH 1:1 solution of sodium methoxide (5 mg) was added to the resin and shaken for 20 hours. Evaporation of the solvent in vacuo gave the desired methyl ester (XXI). The deprotection / cyclization step was performed with acetyl chloride in 10% methanol or trifluoroacetic acid in 10% dichloro...
Embodiment 3
[0558] Solutions of compounds of formula (II) by the '3-step-one-pot' method using the Ugi multi-component reaction Composite
[0559] Using equal amounts (0.1 ml) of 0.1 M solutions of the four appropriate constituent compounds of formulas (XXII), (XV), (XVI) and (IXb), due to 100% conversion, 10 μmol of the final product diketopiperazine is theoretically generated (II). This 4-component condensation was carried out in methanol at room temperature and the solvent was evaporated at 65 °C (using SAVANT _ evaporator for 2 hours). Deprotection / cyclization step with acetyl chloride in 10% methanol or trifluoroacetic acid in 10% dichloroethane, and diethylamine in 5% dichloroethane [Note: 10-15 mg N,N- (Diisopropyl)amino-methylpolystyrene (PS-DIEA) is an excellent binding resin to replace diethylamine]. The solvent is then evaporated at 65°C to give the cyclized product of formula (II).
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