Method for establishing hematopoietic stem cells bank by extracting hematopoietic cells from placenta tissues

A technology of hematopoietic stem cells and placental tissue, applied in the direction of blood/immune system cells, animal cells, tissue culture, etc., can solve the problems of high cost and time-consuming, and achieve the effect of shortening time, reducing the incidence of pollution, and increasing the number

Inactive Publication Date: 2005-03-30
上海厚东生物科技有限公司
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Problems solved by technology

Experiments have proved that umbilical cord blood cells can be expanded tens of thousands of times by using various cell stimulating factors and appropriate culture conditions, but the problem is that this expansion takes time and is expensive, and more importantly, due to the expansion, hematopoietic stem cells Differentiation also occurs, and the results of clinical application show that there is not much difference before and after the expansion of cord blood cells for transplantation

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  • Method for establishing hematopoietic stem cells bank by extracting hematopoietic cells from placenta tissues
  • Method for establishing hematopoietic stem cells bank by extracting hematopoietic cells from placenta tissues
  • Method for establishing hematopoietic stem cells bank by extracting hematopoietic cells from placenta tissues

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Embodiment Construction

[0039] The technical solution involved in the present invention is a method for extracting hematopoietic stem cells from placental tissue to establish a hematopoietic stem cell bank. A method for a hematopoietic stem cell bank, the method is divided into a collection step, a detection step, an extraction step, and a concentration and purification step, wherein the concentration and purification step is:

[0040] mixing the extracted placental tissue cells and hydroxyethyl starch in a ratio of 1:6;

[0041] Centrifuge at 500g for 10 minutes in a centrifuge to remove red blood cells;

[0042] Mix with lymphocyte separation solution in a ratio of 1:4;

[0043] Centrifuge at 1000g for 30 minutes in a centrifuge to remove residual red blood cells and mature granulocytes;

[0044] Collect and wash hematopoietic stem cell populations at different developmental stages;

[0045] Create a stem cell bank.

[0046] Furthermore, the collection step refers to: collecting the umbilical c...

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Abstract

A process for extracting hemopoietic stem cells from the placenta of healthy puerperant to create hemopoistic stem cell bank features that the mechanical, enzyme digestive and monoclonal antibody-magnetic bead method is used to extract blood stem cells from placenta, and concentrate and purify them. Its advantages are high activity and high concentration.

Description

technical field [0001] The invention relates to a method for establishing a hematopoietic stem cell bank belonging to the fields of international patent classification C12N and A61K, in particular to a method for extracting hematopoietic stem cells from placental tissue for establishing a hematopoietic stem cell bank. Background technique [0002] Human fertilized egg cells can develop into embryonic cells in the uterus. These cells have the ability to differentiate into various tissue cells, such as nerve cells, muscle cells, liver cells, blood cells, etc., so these cells are also called pluripotent stem cells . Cells that are directed to differentiate in a certain tissue are also called committed stem cells, such as hematopoietic stem cells and neural stem cells. These cells can continue to differentiate into specific cells or tissues, and blood hematopoietic stem cells can continue to differentiate into red blood cells, white blood cells and platel...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K35/50A61P7/06C12N5/0789
Inventor 周胜利宋剑秋胡玉兰
Owner 上海厚东生物科技有限公司
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