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Peptide immunogen, vaccine made thereof for curing allergic reaction and its preparation method

An immunogen and immunogenic technology, applied in the field of molecular immunology, can solve the problems of insufficient use and the effect of population vaccination remains to be seen.

Inactive Publication Date: 2005-05-18
SHANGHAI INST OF BIOLOGICAL PROD CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, since there are thousands of different types of leukocyte antigen (HLA) among human individuals, there are also many kinds of helper T cell determinant peptides required by different individuals. Wang Changyi designed nearly a hundred kinds of helper T cell Cell determinant peptides may not be enough, and the effect of population vaccination remains to be seen

Method used

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  • Peptide immunogen, vaccine made thereof for curing allergic reaction and its preparation method
  • Peptide immunogen, vaccine made thereof for curing allergic reaction and its preparation method
  • Peptide immunogen, vaccine made thereof for curing allergic reaction and its preparation method

Examples

Experimental program
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Effect test

Embodiment 1

[0101] The chemical synthesis of embodiment 1 human IgE polypeptide and the preparation of liposome vaccine

[0102] (1) Chemical synthesis of peptides

[0103]According to the amino acid residue sequences of the five groups of polypeptides designed in the present invention, A, B, C, D, and E, respectively, on the ABI 431 automatic polypeptide synthesizer (Applied Biosystemes company), according to the operating instructions, carry out solid-phase chemistry one by one. Synthesis (Sheppard RC: Science Tools 1986, 33:9-16). The HMP resin, F-moc series of activated amino acids and main chemical reagents used are all products of ABI Company. After the synthesis is completed, the dry peptide-resin is taken out, and a pre-cooled dissociation mixture (0.75 g of crystalline phenol, di Thioethylene ether 0.25ml, thioanisole 0.5ml, deionized water 0.5ml, trichloroacetic acid 10ml), the amount added is based on 0.1-1.5g peptide resin plus 10ml. The reaction was stirred at room temperat...

Embodiment 2

[0110] Except using KLH (product of Piere, USA) as the carrier protein, the polypeptide-carrier protein was prepared according to the method of Example 1 (2) and (3) and liposome vaccine was further prepared.

Embodiment 3

[0112] Purified TT (semi-finished product of corresponding vaccine) without protective agent was used as carrier protein. Take 2 mg of this carrier protein to prepare a solution: take 2 mg of A-4 polypeptide and C-1 polypeptide (or A-1 polypeptide and E-1 polypeptide, etc.) and dissolve in PBS. After the carrier protein solution was mixed with the mixed polypeptide solution, glutaraldehyde solution was added to make the final concentration 0.2%, and the dipeptide-carrier protein complex was obtained in the same manner as in Example 1 (two), and used in the same manner as in Example 1 ( 3) Vaccines are prepared in the same way.

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Abstract

The invention relates to a peptide immunogen containing human IgE receptor binding site structure, a vaccine for treating allergy made by using the peptide immunogen and a preparation method thereof. The peptide immunogen of the present invention is one or more peptides selected from the five peptide groups of the Cε3 region and Cε4 region of IgE, which are cross-linked with carrier proteins to form immune complexes or made fusion by genetic technology protein, adjuvanted to make a vaccine. After the vaccine is vaccinated in humans or mammals, it can induce the body to produce antibodies that can cross-react with IgE, prevent IgE from binding to receptors, and be used to prevent and treat IgE-mediated allergic diseases.

Description

technical field [0001] The invention belongs to the scope of molecular immunology, relates to a vaccine for treating allergy and its preparation, more specifically relates to a peptide immunogen containing human IgE receptor binding site structure, a vaccine for treating allergy made by using it and a preparation method thereof. Background technique [0002] Type I immediate hypersensitivity in allergy is mediated by IgE molecules. After exposure to allergens, atopic individuals will produce more IgE against allergens. This IgE passes through circulating body fluids and mast cells and basophils in tissues (hereinafter referred to as target cells: target cells) The high-affinity receptor FcεRI on the surface of cells) binds to make these cells "sensitized". When the allergen enters the body again, it will react with the Fab' end of the IgE (cell-bound IgE) bound to the target cell receptor and cause cross-linking, triggering intracellular information ...

Claims

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Application Information

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IPC IPC(8): A61K39/35C07K7/08C07K14/00
Inventor 刘庆良
Owner SHANGHAI INST OF BIOLOGICAL PROD CO LTD
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