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Method of preventing browning in plant tissue culture

A plant tissue culture and tissue culture technology, applied in the field of plant tissue culture, can solve the problems of high toxicity of explants, the inability to popularize new Cattleya varieties quickly, and the inability to effectively overcome the anti-browning technology, and achieve the goal of solving browning Effect

Inactive Publication Date: 2005-06-29
广东省农业科学院花卉研究所
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AI Technical Summary

Problems solved by technology

The browning phenomenon in Cattleya tissue culture is very toxic to the explants, resulting in the abnormal growth and development of the explants, and even the death of the explants
In this regard, the existing anti-browning technology can not be effectively overcome, resulting in the rapid promotion of Cattleya new varieties

Method used

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Examples

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Effect test

Embodiment Construction

[0006] Embodiment (1) get the medium (MS+BA3mg / L+3% sucrose, PH5.8, agar 0.7% solidified), inoculate 25ml of culture medium in each culture bottle, and inoculate one pedicel bud and 3-4 pieces of anthurium buds with a diameter of about 1cm, which can basically eliminate the phalaenopsis pedicel Browning phenomenon in bud tissue culture. The anthurium cluster buds are pre-cultured on the medium for 3 to 5 days, and then the phalaenopsis buds are connected, the effect is better.

[0007] Embodiment (2) The intermediate propagules of Anthurium rapid propagation are first subcultured on the subculture medium (MS+BA3mg / L+NAA0.2mg / L+3% sucrose, pH5.8, agar 0.7% solidified) About 30 days (25ml of culture medium in each culture bottle, inoculate 3 to 4 anthurium buds with a diameter of about 1cm), remove the culture, and then inoculate Cattleya axillary bud explants on the medium, which can basically eliminate The browning phenomenon in the tissue culture of Cattleya axillary buds,...

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Abstract

A method for preventing browning in plant tissue culture. It mainly utilizes the metabolic process of tissue culture of non-browning plants in tissue culture to inhibit the occurrence of browning in plant culture that is prone to browning. The intermediate propagules of rapidly propagating plants that do not brown (hereinafter referred to as the medium propagules) are co-cultured with the explants or cultures of plants that are prone to browning (hereinafter referred to as the culture objects), and the culture objects are inoculated into the medium plants that have been cultured for 20 to 40 days. Days of culture medium and inoculation of the culture object into a medium prepared with a liquid medium that has been cultured can effectively inhibit the occurrence of browning in plant tissue culture that is prone to browning, and even eliminate the browning phenomenon. .

Description

technical field [0001] The invention relates to a plant tissue culture method, in particular to a method for preventing browning in plant tissue culture. Background technique [0002] Browning often occurs in plant tissue culture rapid propagation. This is due to the oxidation of phenolic substances exuded from the surface of explants or culture cuts, which makes the medium dark brown. Browning will cause different degrees of toxicity to explant tissues or cultures of different species, and severe cases will lead to the death of cultures. At present, the measures to reduce browning include: (1) Pretreatment of the mother plant and the explants, such as high temperature or low temperature treatment of the explants, the explants are first pre-cultured on a liquid medium for a few days, and then placed in the Cultivation on semi-solid medium: (2) Select suitable medium and culture conditions. Under suitable medium and culture conditions, the explants will grow vigorously, hav...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 吕复兵
Owner 广东省农业科学院花卉研究所
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