Unlock instant, AI-driven research and patent intelligence for your innovation.

Use of gene recombination human lysozyme against drug resistant bacteria in preparation of medicine

A technology of human lysozyme and gene recombination, which is applied to the genetically recombined human lysozyme, and the genetically recombined human lysozyme in the field of drug-resistant bacteria, and achieves the effects of simple preparation process, abundant sources and good medicinal prospects.

Inactive Publication Date: 2005-11-16
长春奇龙生物技术研究所 +2
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Methicillin-resistant Staphylococcus aureus (MRSA) has no cure other than vancomycin

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] After the culture medium was sterilized by high pressure, the human lysozyme strain was inoculated, the rotation speed of the shaker was 250 rpm, the culture temperature was 20° C., and cultured on a constant temperature bed for 36 hours. The seed tank culture is carried out, and finally the production tank culture is carried out. Extract and purify the culture medium that has been fermented and expressed, freeze-dry the extracted and purified protein concentrate, measure the activity of the protein and store it. Take 1% of human lysozyme freeze-dried powder, phosphate buffer 5mM (pH5.0) 90%, and make injections, which are mainly used for drug-resistant diseases caused by Gram-positive bacteria and Gram-negative bacteria . Such as bronchitis, pneumonia and other diseases.

Embodiment 2

[0029] After the culture medium was autoclaved, inoculated with human lysozyme strain and cultured on a constant temperature bed for 40 hours, the culture medium was purified to make human lysozyme freeze-dried powder containing 0.1%, phosphate buffer 20mM (pH7.0) 80%, 25% glycerol, 5 / 10,000 Tween 80 spray.

Embodiment 3

[0031] After autoclaving the culture medium, inoculate the human lysozyme strain and culture it on a constant temperature bed for 36 hours, then purify the culture medium to make lyophilized powder containing human lysozyme 0.005%, phosphate buffer 10 (pH6.0) Drops of 80%, 20% glycerol, 6% sodium 2-pyrrolidone-5-carboxylate, 0.9% water-soluble azone, 3 / 10,000 Tween 80.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

A genetically recombinant human lysozyme in the form of injection, tablet, capsule, spray dropps and cream for treating the pneumonia, tracheitis, urethritis and cystitis, which are caused by drug-resistant bacteria, is disclosed. It has no toxic by-effect.

Description

Technical field: [0001] The invention relates to a new application of gene recombinant human lysozyme, in particular to the new application of gene recombinant human lysozyme in targeting drug-resistant bacteria in the field of pharmacy. Background technique: [0002] Antibiotics have created many medical miracles and made many diseases disappear without a trace, such as pneumonia, meningitis, puerperal fever, sepsis, tuberculosis, etc. Today in the 21st century, the development of drug-resistant bacteria is shocking. For example, penicillin-resistant Streptococcus pneumoniae was very sensitive to penicillin, erythromycin, sulfonamide and other drugs in the past, but now it is almost "invulnerable". The resistance of Klebsiella pneumoniae to 16 kinds of high-end antibiotics such as Xilixin and Fudaxin is as high as 51.85%-100%. Methicillin-resistant Staphylococcus aureus (MRSA) has no cure except vancomycin. It can be seen from the development history of bacterial drug re...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/47
CPCA61K38/47A61P1/02A61P11/00A61P11/02A61P11/04A61P13/02A61P13/10A61P13/12A61P31/04
Inventor 张华安米安献禄
Owner 长春奇龙生物技术研究所