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Chinese prawn S33 microsatellite label detecting technique

A microsatellite marker, the technology of penaeus chinensis, which is applied to the determination/inspection of microorganisms, biochemical equipment and methods, etc., can solve problems such as specific genetic markers of microsatellite polymorphism maps of shrimp that have not yet been seen, and achieve the effect of simple method.

Inactive Publication Date: 2005-12-28
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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Problems solved by technology

At present, there are no reports on the construction of Chinese shrimp microsatellite polymorphism map, specific genetic markers and pedigree authentication.

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  • Chinese prawn S33 microsatellite label detecting technique

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Embodiment Construction

[0015] The following examples describe in detail the method of the present invention in the Chinese shrimp S33 microsatellite core sequence DNA molecular genetic marker technology. Firstly, extract the genomic DNA of Penaeus chinensis and dilute it for later use; then use the S33 microsatellite core sequence in the genome library of Penaeus chinensis to design specific primers at both ends of the sequence; The genomic DNA of the individual is amplified by PCR, and the PCR product is detected by polyacrylamide gel electrophoresis; the bands that appear in the product are used to analyze the genotype of each individual, and the high genetic variation in the S33 core sequence region of Penaeus chinensis is obtained polymorphism map.

[0016] 1. Extraction of Chinese prawn genomic DNA: Take 100 mg of prawn muscle tissue, cut it into pieces, put it into a 1.5ml centrifuge tube, add 500 μl of pH 8.0 TE solution (10mmol / L Tris-CI, 10mmol / LEDTA), and use a grinding rod grind. Add 25...

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Abstract

A detection technology for the S33 microsatellite marker of Penaeus chinensis: first extract the genomic DNA of Penaeus chinensis; then use the microsatellite sequence containing S33 in the genome library of Penaeus chinensis to design specific primers at both ends of the sequence; and analyze the genome of Penaeus chinensis The DNA was amplified by PCR, and the obtained PCR products were separated by denaturing polyacrylamide gel electrophoresis to obtain a polymorphism map showing a high degree of genetic variation in the S33 core sequence region of Penaeus chinensis. The genotype of each individual of Penaeus chinensis at the S33 locus can be obtained by analyzing the bands of the map. The invention is applicable to detection technologies such as genetic markers, pedigree authentication, and genetic map construction of Chinese prawn populations. The method is convenient, fast and accurate, and can visually detect the genotype of each individual of Penaeus chinensis at the S33 locus.

Description

Technical field [0001] The invention belongs to the Chinese prawn DNA molecular genetic marker technology, and is a technical method for detecting the genetic polymorphism of the Chinese prawn at the S33 site. Background technique [0002] Before the present invention was made, there were similar research reports both at home and abroad, "Quick Screening of Recombinant Positive Clones Containing Microsatellites in Chinese Prawns by PCR Method" and "Chinese Shrimp Microsatellites" published by Xu Peng et al. Screening of DNA" and other papers have reported the construction of a partial genome library of Chinese prawns and the screening of microsatellite sequences, and evaluated the core sequences of microsatellites according to the standards proposed by Weber (1990), but his research Results were limited to the acquisition of microsatellite sequences for research and no further application development. In 1999, the French Institute of Marine Development (IFREMER) found 10 mi...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68
Inventor 刘萍孟宪红孔杰王伟继张庆文费日伟王清印
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI