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Fractionation and processing of oilseed meal

A seed, rapeseed technology

Inactive Publication Date: 2006-01-18
UNIVERSITY OF SASKATCHEWAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In conclusion, the high fiber and high phytate content of canola meal limits its feeder value as a protein source for monogastric animals such as pigs, chickens and fish

Method used

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  • Fractionation and processing of oilseed meal
  • Fractionation and processing of oilseed meal

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Isolation of unroasted Canadian canola flakes

[0044] Oil-extracted canola flakes with hexane were obtained from industrial crushing equipment. This material has not been desolvated or roasted. The flakes were stored in burlap bags and kept in the open air for a minimum of 7 days to allow the hexane to evaporate. The desolvated flakes were crushed to break up larger clumps in the flakes.

[0045] Table 1 : Protein and dry matter content during extraction-dehydration of canola flakes

Embodiment 2

[0047] De-phytate of canola flake extract

[0048] Phytase (Natuphos® 5000, BASF) or FFI phytase (non-commercial enzyme supplied by FinnFeeds International) was diluted in water so that 250 μl aliquots were added to 0, 250, 500, 1000, Phytase equivalents of 2000, 4000, 6000, 8000 and 10000 U. One unit of phytase activity is defined as the amount of enzyme source that liberates 1 micromole of inorganic phosphorus per minute from an excess of sodium phytate at pH 5.5 at 37°C.

[0049] In a conical centrifuge tube, 20 g of unroasted desolvated canola flakes were mixed with 100 ml of 50°C, 0.75% NaCl. Press the slurry to 3000 * centrifuge for 10 minutes. The supernatant was removed and divided into 2 ml aliquots in glass test tubes and placed in a 50°C water bath. After 60 minutes had elapsed, the reaction was quenched by adding 1 ml of ice-cold 1M HCl and vortexing. Keep these samples on ice to ensure that the reaction is stopped. Samples were analyzed for soluble phosphoru...

Embodiment 3

[0052] Heat-induced protein curdling of a de-phytic acid extract of canola flakes.

[0053] Unroasted desolvated canola flakes were processed by extraction-dehydration as described in the Examples. In this case, however, the desolvated flakes were screened through a No. 10 U.S. sieve to remove large aggregates from the feedstock.

[0054] The final extract was placed in a 100L steam pot and the temperature of the extract was raised to 50°C. To this mixture was added a phytase (FFI phytase as described in Example 2) to provide 1500 FTU / kg of raw flake stock. The mixture was stirred continuously with a mechanical stirrer and incubated for 60 minutes to affect the dephytase of the extract. At the end of the phytate period, the temperature of the mixture was increased to 95°C and held at this temperature for 5 minutes. At the end of the heat treatment period, the steam to the pot was turned off and cold water was passed through the pipes. A protein-rich curd was formed on top ...

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Abstract

The present invention relates to a process for the aqueous extraction, fractionation and enzymatic treatment of oilseed materials to generate valued products with no significant low value by-product or waste streams. In particular, the fractionation scheme generates a protein-fibre feed ingredient principally for use with ruminant animals and a second dephytinized high protein fraction. The dephytinized high protein fraction has value as feed ingredient for a variety of species of animals.

Description

technical field [0001] The present invention generally relates to methods for the aqueous extraction, separation and enzymatic treatment of oilseed materials to produce valuable products without significant low value by-products and waste streams. In particular, this separation scheme yields one protein-fiber feed ingredient primarily for ruminants and a second de-phytic acid high protein fraction. The high protein fraction of de-phytic acid has value as a feed ingredient for many species of animals. Background technique [0002] Much effort has been directed towards the development of aqueous processing systems and technologies for the production of high value protein concentrates and protein isolates (>90% protein) from oilseeds such as soybeans. The purpose of all these existing processing systems and technologies is to produce a single very high value protein product. Little or no consideration is given to the value of the non-protein components of the raw material....

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23J1/14A23K1/14A23J3/34A23J3/14A23K1/16A23K1/18A23L11/30
CPCC12Y301/03026A23K1/18A23J1/148A23L1/2113A23K1/1813A23J3/14A23K1/14A23K1/1631A23K10/30A23K20/147A23K50/00A23K50/10A23L11/33
Inventor D·D·梅恩兹R·W·纽柯克H·L·克拉森R·T·泰勒
Owner UNIVERSITY OF SASKATCHEWAN
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