Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Immune stimulated holoside substance of bacterial strain from wood layer hole strain and its application

A technology of immune stimulation and P. clefthoof, which is applied in the direction of medical preparations containing active ingredients, drug combinations, carbohydrate active ingredients, etc., and can solve problems such as difficult identification

Inactive Publication Date: 2006-02-08
KOREA INST OF SCI & TECH +1
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Morphologically diverse and modified strains of Phlomophores spp. are difficult to identify when observed microscopically

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Immune stimulated holoside substance of bacterial strain from wood layer hole strain and its application
  • Immune stimulated holoside substance of bacterial strain from wood layer hole strain and its application
  • Immune stimulated holoside substance of bacterial strain from wood layer hole strain and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment I

[0036] Example I: Cultivation of Phellinus spp

[0037] The strains of each Phellinus species given in Table 1 below were cultured on potato dextrose agar (PDA) at a constant temperature of 28°C for 5-12 days. After inoculation in 450 mL of PD broth, each strain was grown at 28° C. and shaking at 120 rpm for 12 days.

[0038] Table 1

[0039]

Embodiment II

[0040] Example II: Isolation of total DNA

[0041] The SDS-phenol method was used to isolate total DNA from mycelium. Each mycelium cultured in Example I was washed with 20mM EDTA, collected by vacuum filtration with a piece of filter paper (Whatman NO.1), stored in a deep freezer maintained at -70°C and freeze-dried. Grind the freeze-dried mycelium, then add 2g of each powder to 20mL extraction buffer (0.2M Tris·HCl, pH8.5; 0.25M NaCl; 25mM EDTA; 0.5%(w / v)SDS), Then add 14mL phenol and 6mL chloroform. After mixing by slow stirring, the solution was centrifuged. The resulting supernatant was treated with 15 μl RANase (10 mg / mL) at 37° C. for 30 minutes, and then with 15 μl proteinase K (10 mg / mL) at 60° C. for another 15 minutes. Thereafter, the enzyme-treated supernatant was thoroughly mixed with an equal volume of a mixture of phenol, chloroform and isoamyl alcohol (25:24:1), and then centrifuged at 12,000X for 30 minutes at 4°C. Before repeating the same centrifugation, the sup...

Embodiment III

[0042] Example III: Isolation of Mitochondrial DNA

[0043] Ultracentrifugation was performed to separate the mitochondrial DNA from the total DNA obtained from each strain of Example II. After mixing with 8.8 g of cesium chloride (available from Sigma in the United States) and 6 μl of dibenzimidium solution (10 mg / mL), the total DNA solution (8 mL) was ultracentrifuged at 40,000×g at 20°C.

[0044] After further identifying the two DNA bands under ultraviolet light, only the upper band that was the mitochondrial DNA fraction was recovered with the help of a 21-gauge syringe needle. An equal volume of isopropanol saturated with CsCl was added to the DNA fraction thus obtained, and after vortexing the mixture gently, it was centrifuged at 1,200Xg at 4°C. Repeat the washing step 6-7 times in order to completely remove dibenzimine from the mitochondrial DNA fragments. Cesium chloride was removed by adding 70% ethanol three times the volume of the mitochondrial DNA fragment to the mit...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention discloses a new fissiped wood shelf fungus Yoo. A polyose substance separated from the sporophore and mycelium of it or other wood shelf funguses has the anticancer activity caused by stimulating the cellulotoxic action of T-cells and the antibody forming power of B-cells and the activity of resisting cancer transfer. Said polyose can stimulate human immunity to prevent and cure immunopathy, such as cancer and AIDS.

Description

Technical field: [0001] The present invention relates to a strain belonging to the genus Phyllosporium, a polysaccharide substance produced from the strain and the application of the polysaccharide substance. In particular, it relates to a new strain of Phellinus spp., a new polysaccharide substance with effective immunostimulatory activity produced from this strain, and its use in the prevention and treatment of immune-related diseases such as cancer and AIDS and its mechanism Research applications. Background technique: [0002] Mushrooms, a kind of advanced fungi, are heterotrophic organisms; they absorb organic nutrients from the outside through the microscopic hyphae. In the biosphere, they act as a decomposer, degrading complex organic matter into simple inorganic forms and eventually returning to nature. Among the fungi, the most typical mushrooms belong to the class of Basidiomycetes. They characteristically form basidiomycetes, and each basidiomycete will have four exoph...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12P19/04A61K31/715A61K31/70A61P35/00A61P37/02
Inventor 俞益东赵秀默朴柄彧刘载国洪南斗金桓默韩相配李昌雨
Owner KOREA INST OF SCI & TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com