Shaping circular light three differential confocal microscope

Abstract

The invention belongs to optical microscope imaging and microcosmic measurement field, which relates to an integral ring light three-difference cofocal microscope with high signal-to-noise ratio and three-dimension super resolution imaging ability; it mainly includes laser, beam expanding device, polarization spectroscope, lambda / 4 wave plate, microscope lens, gathering lens; and pin apertures and detectors, it also includes a ring light integral device whose incidence light is ring type between the beam expanding device and the polarization spectroscope, adjusts the adjustable diaphragm. The invention improves the transverse resolution of the microscope.

Description

Technical field [0001] The invention belongs to the technical field of optical microscopic imaging and microscopic measurement, and relates to a shaping ring light triple-differential confocal microscope with high performance-to-noise ratio and three-dimensional super-resolution imaging capabilities, which can be used to measure the three-dimensional surface topography and three-dimensional Micro structure, micro step, micro groove, integrated circuit line width, etc. Background technique [0002] The idea of ​​confocal microscope was first proposed by the American scholar M. Minsky in 1957, and was granted a US patent in 1961, with the patent number US3013467. The confocal microscope places the point light source, the point object and the point detector in the corresponding conjugate positions to form a point illumination and point detection microscope imaging system with unique tomographic capabilities in optical microscopy imaging. The basic principles of general confocal micr...

AI Technical Summary

Problems solved by technology

Generally speaking, the above-mentioned new confocal microscope and super-resolution technology have improved the resolution characteristics of the confocal microscope and solved the needs of many confocal microscope super-resolution microscopic imaging measurements, but they still have the following problems: First, the existing Various forms of confocal microscopes use the detected light intensity signal to directly perform imaging processing, which is easily affected by factors such as light intensity fluctuations, background light interference, and environmental temperature drift. The signal-to-noise ratio of the confocal microscope imaging system is low; The axial tomographic accuracy of the confocal microscope is limited by the nonlinearity of the axial intensity response curve, and the existing super-resolution technology tends to cause the increase of the side lobe and the increase of the nonlinear error of the axial response curve during the super-resolution imaging process

However, the three-differential confocal microscopy imaging method is mainly used to improve the axial resolution of the confocal microscope, and does not improve the lateral resolution. When the existing super-resolution pupil filter is used for three-dimensional super-resolution measurement of the confocal microscope, both It is necessary to perform horizontal super-resolution and also take into account axial super-resolution. The effect of three-dimensional super-resolution is usually not particularly significant.

Images