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Method for quickly reproducing engineering seedling of myriophyllum

A technology of foxtail algae and engineering, applied in the field of rapid production of foxtail algae high-quality engineering seedlings, can solve problems such as limited source of foxtail algae, destruction of original habitat, root system damage, etc., to achieve the possibility of avoiding genetic variation and low cost , the effect of convenient operation

Inactive Publication Date: 2006-09-06
NANJING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The stem of the plant is soft and multi-branched, and the root system was severely damaged during the salvage process, and the size of the plants varies, which is not conducive to its successful transplantation
In addition, natural sources of foxtail algae contain various algae and miscellaneous bacteria, which is not conducive to the safe use in fish, shrimp and crab ponds and indoor ornamental aquariums
Finally, the source of foxtail algae in the natural environment is limited, and excessive collection will easily lead to the destruction of its original habitat
[0004] So far, there have been no researches and reports on the cultivation and large-scale artificial propagation of foxtail algae aseptic vaccines at home and abroad

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0023] Example 1. In October, 2003, the provenance of Trichosanthes was collected from Jiangpu County, Nanjing, and 5 bud segments with a length of 2 cm were cut, soaked in soapy water for 4 hours, and then rinsed with tap water. Enter the ultra-clean studio, soak in 70% ethanol for a few seconds, then soak in NaClO bleach containing a certain amount of Tween-80 diluted in an appropriate ratio for a few minutes, rinse thoroughly with sterile water, and inoculate each bud individually The culture medium ① was placed in a sterilized sealed Erlenmeyer flask and cultivated in the tissue culture room. The average light intensity is 2000lx, the daily light is 10h, and the temperature is about 25°C. Afterwards, after observation, it was found that 2 bottles were still contaminated, while the other 3 bottles were sterile and began to turn green obviously after a few days. After 2 weeks, in the ultra-clean studio, the lateral buds above 2 cm in each of the 3 bottles of material were c...

example 2

[0024] Example 2. In November, 2003, the source of the foxtail algae was collected from East Taihu Lake, Suzhou, and 20 bud segments with a length of 2 cm were cut, soaked in soapy water for 4 hours, and then rinsed with tap water. Enter the ultra-clean studio, soak in 70% ethanol for a few seconds, and then continue to disinfect with NaClO bleach diluted in an appropriate ratio and containing a certain amount of Tween-80. After thoroughly rinsing with sterile water, inoculate each bud individually in the culture medium ① and cultivate in the tissue culture room. The average light intensity is 2000lx, the daily light is 10h, and the temperature is about 25°C. A total of 11 contaminated materials were removed within 2 weeks, and 5 of the other 9 materials increased significantly. A total of 48 lateral buds were obtained after the initial cutting, which were continued to be cultured in the culture medium ①. Thereafter, they were divided every 2 weeks and transferred to new med...

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Abstract

A method for fast reproduction of the engineering myriophyllum features use of tissue culture method and obtaining large-scale high-quality engineering seedling.

Description

1. Technical field [0001] The invention belongs to the field of biotechnology, and relates to a method for rapidly producing high-quality engineering seedlings of foxtail algae. 2. Background technology [0002] Myriophyllum spicatum Linn. is a kind of aquatic herbaceous plant of Myriophyllum spicatum Linn., which grows in ponds, lakes or rice fields. The plant has a high tolerance to water pollution, and is often used as a water purification tool species and a vegetation restoration pioneer species in eutrophic water ecological restoration projects. The plant is also widely planted in fish, shrimp and crab ponds, which can not only provide bait for the above-mentioned aquatic products, but also provide them with necessary refuge and spawning places. In addition, the plant is also a commonly used landscaping material in the process of indoor ornamental aquaculture. [0003] At present, there is a great demand for foxtail algae seedlings in the market. However, the researc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G33/00A01H4/00A01K61/00C02F3/32
CPCY02A40/81
Inventor 周长芳安树青尹大强蒋金辉
Owner NANJING UNIV
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