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Mycobacteria detecting method and device

The technology of a mycobacteria and a detection method is applied in the field of detection of mycobacteria, and can solve the problems of hazards to operators, failure of experiments, carcinogenicity of xylene, etc., and achieves improved detection rate, simple detection device and equipment, and simple steps. less effect

Inactive Publication Date: 2006-09-06
黄伟
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The disadvantages of this detection method are: the positive rate of the detection result is low, the operation is easy to cause misoperation, which leads to the failure of the experiment, and xylene is carcinogenic, which is harmful to the operator

Method used

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  • Mycobacteria detecting method and device
  • Mycobacteria detecting method and device
  • Mycobacteria detecting method and device

Examples

Experimental program
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Effect test

Embodiment 1

[0022] like figure 1Shown is the flow chart of the mycobacterium detection method of the present invention, in the 10 steps of sterilizing the mixed solution of the sample liquid and the diluent, at first select 5ml of sputum sample liquid not containing mycobacteria through autoclaving, Add 0.5ml of the prepared mycobacteria standard solution to the sample solution, and the mycobacteria contained in it will be 400-600cfu, then add a diluent concentration of 0.9% sodium chloride solution to 20ml, and high-pressure steam 0.105 Mpa, sterilized for 20 minutes; when sterilizing, a microporous membrane can be covered on the top of the sample solution to prevent false positives caused by mycobacterial contamination during sterilization; then perform 20 steps of extraction, and in the sterilized sample containing Add 3ml of extractant to the mixture of liquid and sodium chloride diluent for ultrasonic extraction, the extractant is composed of butyl acetate and ether, the mixing ratio...

Embodiment 2

[0025] like figure 1 Shown is the flow chart of the mycobacterium detection method of the present invention. In the 10 steps of sterilizing the mixed solution of the specimen liquid and the diluent, at first select 5ml of sputum specimens that do not contain mycobacteria through autoclaving, and then Add 1.0ml of the prepared mycobacteria standard solution to the standard solution, and the mycobacteria contained in it will be 800-1200cfu, then add a sodium chloride solution with a diluent concentration of 0.9% to 20ml, and a high-pressure steam of 0.105 Mpa, sterilized in 20 minutes; when sterilizing, a microporous membrane can be covered on the top of the sample solution to prevent false positives from the contamination of mycobacteria during sterilization; in the extraction step 20, its operating steps are the same as in Example 1 The same, but the difference is that the mixing ratio of the added extractant butyl acetate and ether is 100:17, and the ultrasonic generator with...

Embodiment 3

[0028] like figure 1 Shown is the flow chart of the mycobacterium detection method of the present invention. In the 10 steps of sterilizing the mixed solution of the specimen liquid and the diluent, at first select 5ml of sputum specimens that do not contain mycobacteria through autoclaving, and then Add 3.0ml of mycobacterium standard solution prepared above to the standard solution, then the mycobacteria contained in it is 2400-3600cfu, then add sodium chloride solution with a diluent concentration of 0.9% to 20ml, and high-pressure steam 0.105 Mpa, sterilized in 20 minutes; when sterilizing, a microporous membrane can be covered on the top of the sample solution to prevent false positives from the contamination of mycobacteria during sterilization; in the extraction step 20, its operating steps are the same as in Example 1 The same, but the difference is: the mixing ratio of the added extractant butyl acetate and ether is 100:50, and the ultrasonic generator with a frequenc...

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Abstract

This invention relates to a detecting method and a checkout instrument about mycobacterium. The processes includes that at first diluting specimen liquid, disinfecting, abstracting, then stewing it for spell of time to deliver liquid, dry, acid-fasting stain and examine the result by microscope. The instrument comprises three parts including chamber of ultrasonic extraction, system of automatic delivering liquid, specimen drying chamber. It can accurately detect the number of mycobacterium in the specimen liquid, and improves the detectable rate ten times higher than method of float collecting bacterium and has important meaning not only in the diagnosis, prevention and cure of tuberculosis but also in the differential diagnosis of many clinical symptoms.

Description

technical field [0001] The invention relates to a method for detecting mycobacteria, and at the same time relates to a detection device for realizing the method for detecting mycobacteria. Background technique [0002] Mycobacteria include Mycobacterium tuberculosis, Nontuberculous mycobacteria, Bacillus leprae and other bacteria. Mycobacterium tuberculosis can cause human tuberculosis, there are Mycobacterium tuberculosis and Mycobacterium bovis. Tuberculosis has the characteristics of many patients and great harm, and it also causes psychological pressure on the patients themselves and their families. In recent years, the incidence rate has increased significantly, which has attracted the attention of the government departments and adopted corresponding compulsory measures. The medical community has done a lot of research on it and developed many detection methods. [0003] Symptoms and signs of tuberculosis are often atypical. Although X-rays can be used for diagnosis, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/04C12R1/32G01N33/569
Inventor 黄伟徐进王域平张伟邵永生
Owner 黄伟
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