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Culturing system of external light biological reactor for plant tissue cutter

A photobioreactor and bioreactor technology, applied in the field of culture systems, can solve the problems of inability to realize continuous long-term photobioreactor cultivation, increase the microbial contamination rate, cutting work, and affect the smooth progress of experiments, and achieve easy independent operation, Improve the effect of scientificity and easy disassembly and cleaning

Inactive Publication Date: 2007-03-07
SHANGHAI JIAO TONG UNIV
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Problems solved by technology

Its shortcoming is that this system can only provide staged culture for micropropagules, and cannot realize continuous long-term photobioreactor cultivation. In addition, the system can only cut micropropagules off-line, which is greatly improved. Increased the microbial contamination rate and tedious cutting work during the cultivation process, which brings inconvenience to the experiment; when applied to large-scale cultivation, offline cutting becomes a limiting step, which seriously affects the smooth progress of the experiment

Method used

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  • Culturing system of external light biological reactor for plant tissue cutter
  • Culturing system of external light biological reactor for plant tissue cutter
  • Culturing system of external light biological reactor for plant tissue cutter

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Embodiment Construction

[0025] As shown in Figures 1-8, the present invention includes: infusion system 1, gas delivery system 2, illumination system 3, bioreactor cultivation system 4, data receiving processor 5, online photosensitive monitor 6, tee device 7, cutting System 8, first air filter 9, vacuum / pressure dual purpose pump 10. The connection method is as follows: the infusion system 1 is connected to the bioreactor cultivation system 4 through a silicone tube; the gas delivery system 2 is connected to the bioreactor cultivation system 4 through a silicone tube; the lighting system 3 is fixed on the periphery of the bioreactor cultivation system 4; The photosensitive monitor 6 is installed on one side of the photobioreactor cultivation system 4, and transmits the signal to the data receiving processor 5; the three ends of the three-way device 7 are respectively connected to the two interfaces and the cutout of the bioreactor cultivation system 4 through silicone tubes. The interface of the sys...

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Abstract

An outlay plant tissue cutter optical bioreactor culture system which contains transfusion system, air transportation system, illumination system and bioreactor culture system, data processor, light-sensitive monitor, tee valve, incision system, air filter, vacuum / pressure pump. The silicone tube is the connection of the transfusion system and air transportation system to the bioreactor culture system; the outside of it is illumination system; and the other side is light-sensitive monitor. The invention can decrease the pollution of the incision greatly.

Description

technical field [0001] The invention relates to a culture system, in particular to a plant tissue cutter external photobioreactor culture system. Used in the field of plant tissue engineering. Background technique [0002] The research on photobioreactors began with the large-scale cultivation of microalgae in the 1940s, and has developed rapidly since the 1980s. So far, a wide variety of photobioreactors have been developed, and the pH value can be inserted into the photobioreactor. Electrode, dissolved oxygen electrode, temperature electrode, cell density electrode, viscosity electrode, etc., the signals obtained by the above sensors can be further converted to realize the programmed automatic recording and control of the photobioreactor. The land plant and large seaweed cells or tissues used for cultivation are mainly in the form of callus, micropropagule or gametophyte cells, which have a common feature of strong growth and expansion capabilities, and can continue to gr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/00C12M3/00
CPCC12M31/02C12M21/02C12M21/08C12M41/06C12M27/02
Inventor 张宝红齐瀚实赵锐陈思晔强静
Owner SHANGHAI JIAO TONG UNIV
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