Process for producing casein peptide using coarse cheese as raw material
A blood pressure-lowering peptide and crude cheese technology, applied in cardiovascular system diseases, fermentation, drug combination and other directions, can solve the problems of increasing the bitterness of hydrolyzates, increasing free amino acids, and reducing the amount of peptides produced, so as to improve the economic benefits of pastoral areas and delay the arterial Hardened, low-cost effect
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Embodiment 1
[0026] Using coarse cheese as a raw material, under the conditions of feeding mass concentration of 10% and temperature of 42°C, adjusting the pH value between 6-10 and dissolving for 70 minutes. Then the dissolved emulsion is mechanically degreased, deodorized by a vacuum deodorizer, and then 5% hydrochloric acid is used to precipitate and separate the casein, and the separated casein is dissolved again. Conditions, adjust the pH value between 2-9, dissolve for 50 minutes. Then, the dissolving emulsion is enzymatically hydrolyzed with papain. During the enzymolysis process, the temperature is controlled at 38° C., the pH value is at 2-9, and the enzymolysis is carried out for 100 minutes. Then use 2N hydrochloric acid to terminate the reaction, separate and remove undecomposed protein, desalt by ultrafiltration, and spray (freeze) dry to obtain the finished casein hypotensive peptide.
Embodiment 2
[0028] Edible casein is used as a raw material, under the conditions of feeding weight concentration of 8% and temperature of 34 DEG C, adjusting the pH value between 2-9 and dissolving for 50 minutes. Then the dissolved emulsion is enzymatically hydrolyzed with pepsin. During the enzymolysis process, the temperature is controlled at 34° C. and the pH value is 2-9, and the enzymolysis is carried out for 60 minutes. Then use 1N hydrochloric acid to terminate the reaction, separate and remove the undecomposed protein, desalt by ultrafiltration, and spray (freeze) dry to obtain the finished casein hypotensive peptide.
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