Fungal cells with inactivated DNA mismatch repair system

A technology of mismatch repair and DNA sequencing, applied in DNA preparation, recombinant DNA technology, fungi, etc., can solve the problem of library diversity limitation
CN1341146AInactive Publication Date: 2002-03-20NOVOZYMES AS
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
NOVOZYMES AS
Publication Date
2002-03-20
Estimated Expiration
Not applicable · inactive patent

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Abstract

A process for making DNA libraries in filamentous fungal cells using a novel cloned gene involved in the mismatch repair system of filamentous fungal cells.
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Description

field of invention

[0001] A method for preparing DNA libraries of filamentous fungal cells using novel cloned genes involved in the mismatch repair system of filamentous fungal cells. Background of the invention

[0002] The mismatch repair system is a system within a cell that recognizes mismatches in newly synthesized double-stranded DNA sequences.

[0003] Mismatch repair systems can then correct mismatches perceived as errors using the methylated "old" strain as a template, or can mediate degradation of the double-stranded DNA sequence containing the mismatch.

[0004] Independent of the precise mechanism, the end result would be that the "mismatch repair system" would limit the "diversity" within the cell, which manifests itself as double-stranded DNA sequences containing mismatches.

[0005] For example, a double-stranded DNA sequence containing a single mismatch represents a diversity of two different DNA sequences within the cell. If the m...

Claims

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