Stem cell growth factor-like polypeptides

a stem cell growth factor and polypeptide technology, applied in the field of new stem cell growth factorlike polypeptides, can solve the problems that stem cells in general are extremely difficult to culture and maintain in vitro, and achieve the effect of reducing the side effects of such an agen

Inactive Publication Date: 2004-01-06
KIRIN PHARMA +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Stem cells in general have been extremely difficult to culture and maintain in vitro, let alone directing them on a predetermined differentiation pathway.

Method used

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  • Stem cell growth factor-like polypeptides
  • Stem cell growth factor-like polypeptides
  • Stem cell growth factor-like polypeptides

Examples

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example 1

Isolation of SEQ ID NO:1-21 from a cDNA Libraries of Human Cells

A plurality of novel nucleic acids were obtained from a cDNA library prepared from human fetal liver spleen, ovary, adult brain, lung tumor, spinal cord, cervix, ovary, endothelial cells, umbilical cord, lymphocyte, lung fibroblast, fetal brain, and testis, using standard PCR, sequencing by hybridization sequence signature analysis, and Sanger sequencing techniques. The inserts of the library were amplified with PCR using primers specific for vector sequences flanking the inserts. These samples were spotted onto nylon membranes and interrogated with oligonucleotide probes to give sequence signatures. The clones were clustered into groups of similar or identical sequences, and single representative clones were selected from each group for gel sequencing. The 5' sequence of the amplified inserts was then deduced using the reverse M13 sequencing primer in a typical Sanger sequencing protocol. PCR products were purified and...

example 2

Assemblage of SEQ ID NO: 22 and 24

The novel nucleic acids (SEQ ID NO: 22 and 24) of the invention were assembled from sequences that were obtained from a cDNA library by methods described in Example 1 above. The final sequences were assembled using the EST sequences as seed. Then a recursive algorithm was used to extend the seed into an extended assemblage, by pulling additional sequences from Hyseq's database containing EST sequences that belong to this assemblage. The algorithm terminated when a complete contig was assembled. Inclusion of component sequences into the assemblage was based on a BLASTN hit to the extending assemblage with BLAST score greater than 300 and percent identity greater than 95%.

The nearest neighbor result for the assembled sequence (SEQ ID NO. 22 or 24) was obtained by a FASTA version 3 search against Genpept release 114, using Fastxy algorithm. Fastxy is an improved version of FASTA alignment which allows in-codon frame shifts. The nearest neighbor result ...

example 3

Assemblage of SEQ ID NO: 27

The novel nucleic acid (SEQ ID NO: 27) of the invention was initially assembled from sequences that were obtained from a cDNA library by methods described in Example 1 above. The final sequence was assembled using the EST sequences as seed. Then a recursive algorithm was used to extend the seed into an extended assemblage, by pulling additional sequences from Hyseq's database containing EST sequences that belong to this assemblage. The algorithm terminated when a complete contig was assembled. Inclusion of component sequences into the assemblage was based on a BLASTN hit to the extending assemblage with BLAST score greater than 300 and percent identity greater than 95%.

Using this initial sequence, suitable primers were designed for amplification of ESTs that comprise the initial sequence. The products were cloned. The DNA was isolated, cut with appropriate restriction enzymes, ligated, and recloned to generate the full-length contig. The full-length produc...

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Abstract

The invention provides novel polynucleotides and polypeptides encoded by such polynucleotides and mutants or variants thereof that correspond to a novel human and mouse secreted stem cell growth factor-like polypeptide. These polynucleotides comprise nucleic acid sequences isolated from cDNA libraries prepared from mouse bone marrow and human fetal liver spleen, ovary, adult brain, lung tumor, spinal cord, cervix, ovary, endothelial cells, umbilical cord, placental, lymphocyte, lung fibroblast, fetal brain, and testis. Other aspects of the invention include vectors containing processes for producing novel human secreted stem cell growth factor-like polypeptides, and antibodies specific for such polypeptides.

Description

1. BACKGROUND1.1 Technical FieldThe present invention provides novel polynucleotides and proteins encoded by such polynucleotides, along with uses for these polynucleotides and proteins, for example in therapeutic, diagnostic and research methods. In particular, the invention relates to a novel stem cell growth factor-like polypeptide.1.2 Background ArtIdentified polynucleotide and polypeptide sequences have numerous applications in, for example, diagnostics, forensics, gene mapping, identification of mutations responsible for genetic disorders or other traits, to assess biodiversity, and to produce many other types of data and products dependent on DNA and amino acid sequences. Proteins are known to have biological activity, for example, by virtue of their secreted nature in the case of leader sequence cloning, by virtue of their cell or tissue source in the case of PCR-based techniques, or by virtue of structural similarity to other genes of known biological activity. It is to the...

Claims

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Application Information

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Patent Type & Authority Patents(United States)
IPC IPC(8): C07K14/435C07K14/475A61K38/00C12N5/08
CPCC07K14/475A61K38/00A61K2039/53Y10S435/81Y10S435/975
Inventor NISHIKAWA, MITSUOLABAT, IVANDRMANAC, RADOJE T.TANG, Y. TOMCHAO, CHENG-CHIMIZE, NANCYCHILDS, JOHN
Owner KIRIN PHARMA
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