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Method for utilization of biological reaction device specially used for variety development of silkworm

A technology of bioreactors and breeds, which is applied in the field of breeding animals or new animal breeds, can solve the problems that the products are difficult to industrialize and produce stably throughout the year, and the potential of silkworm bioreactors cannot be fully utilized, so as to achieve the effect of getting rid of dependence

Inactive Publication Date: 2003-05-21
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The expression level of exogenous genes is affected by nutrients, breeding environment, including microorganisms, and is also affected by seasonal climate. It is difficult for the product to be produced stably throughout the year.
At the same time, since there are no special silkworm varieties for bioreactors for exogenous gene expression at home and abroad, the potential of silkworms as bioreactors cannot be fully utilized.

Method used

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  • Method for utilization of biological reaction device specially used for variety development of silkworm
  • Method for utilization of biological reaction device specially used for variety development of silkworm

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Select silkworm eggs of the middle silkworm variety with large individual body to use Co 60 Radiation is carried out, and the radiation dose is 2000 roentgens.

[0020] The ant silkworms hatched after radiation treatment were fed with low-cost artificial feed designed according to the nutrition and physiology of silkworms, and individuals with mutant feeding habits were screened in the population, and individuals who could complete the generation were selected for random mating and subsequent generation.

[0021] In the process of subculture, the feed formula should be properly adjusted, so that the selected material silkworm can adapt to the feed formula, and the low-cost artificial feed formula can be improved. After four generations of feeding, the large pupae gene was introduced, and the individuals with large pupae were selected for mating among the offspring, and then the NPV susceptibility gene was introduced, and the silkworms in 1 / 2 of the eggs in the moth area...

Embodiment 2

[0023] The Japanese variety SJ was selected and divided into silkworm eggs of Japanese silkworm varieties as raw materials for Co 60 3000 roentgens were subjected to radiation treatment, and the rest were bred with the CBB breeding method.

[0024] The silkworm species bred by this method are specially used for bioreactor silkworms. By ingesting wide-feeding and low-cost artificial feeds, they can implement industrialized silkworm breeding throughout the year. They are fed with sterile artificial feeds and used as the main type of bioreactor in the aseptic breeding room. Stable expression of exogenous gene products.

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Abstract

The present invention discloses a bio-reactor special-purpose silkworm breeding method includes the following steps: using Co60 to radiate silkworm egg to make it undergo the process of radiation-induced mutation treatment, using special-purpose feed to feed ant silkworm, screening, mutant to form euryphagic silkworm breeding source material, introducing said breeding source material into large chrysalis gene and NPV susceptible gene, and adopting multi-generation breeding and isozygoty so as to obtain the invented euryphagic host bio-reactor special-purpose silkworm variety. Said ivnention can change silkworm food habit, and can make silkworm dispense with dependence on unique feed-mulberry leaf.

Description

technical field [0001] The invention relates to a breeding method for animals or new animal varieties, in particular to a breeding method for special silkworm varieties for bioreactors. Background technique [0002] Bioreactor is a hotspot of biotechnology research at home and abroad in recent years. The living body is used as a reactor, and the metabolic pathway of the living body is used under normal temperature and pressure to produce products that cannot be produced by traditional industries or can be produced by traditional industries but are expensive. Benefits and social benefits are immeasurable. The advantage of using insects as bioreactors to express exogenous gene products is that the yield of expression products is high, the expression products with biological activity can be directly formed in insects, and the environment of exogenous genes is safe and easy to control. Bombyx mori is the most ideal bioreactor for expressing foreign gene...

Claims

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Application Information

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IPC IPC(8): A01K67/04
Inventor 计东风孟智启陈诗
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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