Double phase blood substratum capable of making colony coloration

Abstract

The present invention relates to the domain of medical test technology, and is one kind of double phase blood substratum capable of making colony colorating. Into solid phase substratum, redox indicator tetrazolium red is added, so that the place with growing bacteria, exhibits carmine owing to the redox during bacteria growth; and nucleic acid and sodium glutamate in 0.2% concentration are also added to promote the growth of bacteria. The fast bacteria growth and the clony coloration make the present invention capable of detecting fast and simply in timely diagnosis.

Description

Technical field: [0001] The invention relates to the technical field of medical examination, and is a biphasic blood culture medium capable of developing color of bacterial colonies. Background technique: [0002] Blood culture is one of the most important items in clinical microbiological examination. Bacteria entering the blood may cause bacteremia and sepsis, so biphasic blood cultures are usually performed on patients with fever under investigation and surgical infection. The so-called biphasic blood culture means that there are both liquid phase medium and solid phase medium in the culture bottle. First, the blood is injected into the liquid phase medium and gently shaken to infiltrate the surface of the solid phase medium, and then the conventional culture is carried out. If bacteria grow, pick colonies directly from the solid surface, carry out smear staining, biochemical identification and drug susceptibility test, so that targeted antibacterial treatment can be giv...

AI Technical Summary

Problems solved by technology

However, the solid surface of the existing biphasic blood culture is close to colorless, it is difficult to identify tiny colonies, and it is easy to miss detection, which not only makes the positive detection rate of bacteria low, but also may cause misdiagnosis due to "false negative"