Carotenoid possessing anticancer function and its preparing method
A technology of carotene and action, applied in the direction of antineoplastic drugs, organic chemistry, drug combination, etc., can solve the problems of uncertainty, insufficient pharmacological effects of anti-cancer and cancer treatment, low dosage, etc., and achieve significant inhibitory effect, solvent Recyclability, effect of reducing MDA content
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Embodiment 1
[0023] Take the raw material red duck egg, the Roche chroma of the yolk of the red duck egg reaches 19 degrees, after routine cleaning and disinfection, the egg yolk is separated from the egg white, and freeze-dried to obtain freeze-dried egg yolk powder for later use. Take 100 grams of freeze-dried egg yolk powder and use petroleum ether: acetone (2:1V / V) mixed solvent extraction 3 times, each time with 400 ml of mixed solvent to extract for 60 minutes, separate the extract, add 20 ml of distilled water, let stand, make petroleum ether and acetone layered, release the acetone layer, distilled water Wash 2 to 3 times, 15 ml each time, release the water layer, add 3 g of anhydrous sodium sulfate to the petroleum ether layer, recover the petroleum ether layer, obtain the extract containing carotenoids, and concentrate the extract in vacuum at 40°C , put the concentrated extract on a silica gel column, first use petroleum ether: acetone mixture (24:1V / V) to elute non-carotenoids, ...
Embodiment 2
[0025] Take the raw material red duck egg, the Roche chroma of the yolk of the red duck egg reaches 15 degrees, after routine cleaning and disinfection, the egg yolk is separated from the egg white, and freeze-dried to obtain freeze-dried egg yolk powder for later use. Take 100 grams of freeze-dried egg yolk powder and use petroleum ether: acetone (1:1V / V) mixed solvent extraction 3 times, each time with 300 ml of extract, extract for 50 minutes, separate the extract, add 15 ml of distilled water, let stand, make petroleum ether and acetone layered, release the acetone layer, Wash with distilled water 2 to 3 times, 20 ml each time, release the water layer, add 4 grams of anhydrous sodium sulfate to the petroleum ether layer, recover the petroleum ether layer, and obtain the extract containing carotenoids, and vacuum the extract at 40 ° C. Concentrate, put the concentrated extract on a silica gel column, first use petroleum ether: acetone mixture (24:1V / V) to elute non-carotenoi...
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