Use of gene recombination human lysozyme against drug resistant bacteria in preparation of medicine

A technology of human lysozyme and gene recombination, which is applied in the field of gene recombination human lysozyme, and the field of genetic recombination human lysozyme

Inactive Publication Date: 2003-09-03
长春奇龙生物技术研究所 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Methicillin-resistant Staphylococcus aureus (MRSA) has no cure other than vancomycin

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] The recombinant human lysozyme was sterilized by autoclaving, the rotation speed of the shaker was 250 revolutions per minute, and the cultivation temperature was 20°C, and it was cultivated on a constant temperature bed for 36 hours. The seed tank culture is carried out, and finally the production tank culture is carried out. Extract and purify the culture medium that has been fermented and expressed, freeze-dry the extracted and purified protein concentrate, measure the activity of the protein and store it. Take 1% of human lysozyme freeze-dried powder, phosphate buffer 5mM (pH5.0) 90%; make injections, mainly used for drug-resistant diseases caused by Gram-positive bacteria and Gram-negative bacteria . Such as bronchitis, pneumonia and other diseases. Example 2:

Embodiment 2

[0059] After the recombinant human lysozyme was sterilized by high pressure and cultured on a constant temperature bed for 40 hours, the culture solution was purified to make lyophilized powder containing human lysozyme 0.1%, phosphate buffer 20mM (pH7.0) 80%, Spray of 25% glycerol, 5 / 10,000 Tween 80.

Embodiment 3

[0061] After the recombinant human lysozyme was sterilized by high pressure and cultured on a constant temperature bed for 36 hours, the culture solution was purified to make lyophilized powder containing human lysozyme 0.005%, phosphate buffer 10 (pH6.0) 80%, Drops of 20% glycerol, 6% sodium 2-pyrrolidone-5-carboxylate, 0.9% water-soluble azone, and 3 / 10,000 Tween 80.

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PUM

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Abstract

A genetically recombinant human lysozyme in the form of injection, tablet, capsule, spray dropps and cream for treating the pneumonia, tracheitis, urethritis and cystitis, which are caused by drug-resistant bacteria, is disclosed. It has no toxic by-effect.

Description

Technical field: [0001] The invention relates to a new application of gene recombinant human lysozyme, in particular to the new application of gene recombinant human lysozyme in targeting drug-resistant bacteria in the field of pharmacy. Background technique: [0002] Antibiotics have created many medical miracles and made many diseases disappear without a trace, such as pneumonia, meningitis, puerperal fever, sepsis, tuberculosis, etc. Today in the 21st century, the development of drug-resistant bacteria is shocking. For example, penicillin-resistant Streptococcus pneumoniae was very sensitive to penicillin, erythromycin, sulfonamide and other drugs in the past, but now it is almost "invulnerable". The resistance of Klebsiella pneumoniae to 16 kinds of high-end antibiotics such as Xilixin and Fudaxin is as high as 51.85%-100%. Methicillin-resistant Staphylococcus aureus (MRSA) has no cure except vancomycin. It can be seen from the development history of bacterial drug re...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/47
CPCA61K38/47A61P1/02A61P11/00A61P11/02A61P11/04A61P13/02A61P13/10A61P13/12A61P31/04
Inventor 张华安米安献禄
Owner 长春奇龙生物技术研究所
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