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A polypeptide chip agent box and uses thereof

A peptide chip and kit technology, applied in the field of kits, can solve the problem of high price of fluorescence scanners, and achieve the effects of saving detection operation costs, obvious economic advantages, and rapid detection.

Inactive Publication Date: 2003-12-31
CHENGDU KUACHANG SCI & TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In order to overcome the disadvantages of expensive fluorescent scanners, researchers have made some improvements, using colloidal gold and silver colorants to track target molecules, and detecting them with CCD devices or digital cameras (WO 00 / 72018 A1)
However, its focus is still on gene chips, which are only applied to high-density chips with more than 20 distinguishable samples per square centimeter. As a result, high-precision sample printing equipment and special signal detection equipment are still required.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0013] Example 1: Production and application of HIV gold and silver staining chip kit

[0014] In this example, the preparation of the probe plate of the HIV gold and silver staining chip kit is as follows: use the activated microscope slide as the carrier, HIV1+2 antigen as the probe, manually spot the samples, 6 probe spots in 2×3 Arranged in dot matrix, the diameter of each probe point is 1mm, and the distance between probe points is 3mm. After the probe is fixed, the uncoated part is blocked by the blocking solution, and then dried for later use.

[0015] The labeling system of the HIV gold and silver staining chip kit is colloidal gold-labeled goat anti-human IgA and silver nitrate solution.

[0016] An example of the application of the HIV gold and silver staining chip kit to the rapid detection of HIV: first add the serum sample to be tested, after the reaction is completed, wash off the unbound serum components, add colloidal gold-labeled secondary antibody, when the ...

example 2

[0017] Example 2: Production and application of hepatitis C gold and silver staining chip kit

[0018] In this example, the preparation of the probe plate of the gold and silver staining chip kit is as follows: using activated microscope slides as carriers, hepatitis C NS3, NS4, and CORE antigens as probes, manual spotting, each probe spot 6 Probe points are arranged in a 3×6 dot matrix, each probe point has a diameter of 1mm, and the distance between the probe points is 3mm. After the probe is fixed, the uncoated part is blocked by the blocking solution, and then dried for later use.

[0019] The labeling system of Hepatitis C Gold and Silver Staining Chip Kit is colloidal gold-labeled goat anti-human IgA and silver nitrate solution.

[0020] An example of hepatitis C gold and silver staining chip kit applied to hepatitis C detection: first add the serum sample to be tested, after the reaction is completed, wash off the unbound serum components, and then add a colorant-label...

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PUM

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Abstract

The present invention provides one kind of polypeptide chip reagent kit with relatively low requirement in hardware and software for printing, scanning and processing scanning result. The present invention features lower distribution density of probes in selective reactor on the probe plate, less than 20 each square centimeter; and the labeling system with high sensitivity colloid metal, silver salt or other coloring agent as labeling matter. The chip reagent kit is especially suitable for use in detection with less destination molecules.

Description

technical field [0001] The invention relates to a kit for qualitative and / or quantitative detection of target compounds in biological samples, in particular to a low-density polypeptide chip probe board and colloidal metal and silver salt labeling substances as the main components. The result can be directly judged by the naked eye or by an instrument. [0002] The present invention also relates to the manufacturing method and application of the above polypeptide chip kit. Background technique [0003] A biochip is a qualitative and / or quantitative detection product characterized by immobilizing microprobes on the surface of a solid phase carrier in a non-random manner, such as an addressable manner. Then, the immobilized probe can specifically react with the target molecule in the biological sample under the condition of micro-detection, and the result of the specific reaction can be marked and read out. According to the different probes immobilized on the carrier, biochi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68G01N33/543G01N33/569G01N33/68
Inventor 邹方霖陈春生胡劲梅王建霞
Owner CHENGDU KUACHANG SCI & TECH CO LTD
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