Method and device for detecting and identifying bio-aerosol particles in air

A bioaerosol and particle technology, applied in the direction of measuring devices, particle and sedimentation analysis, parts of particle separator tubes, etc., can solve unsatisfactory, insensitive bioaerosol particles and other problems
CN1481575AInactive Publication Date: 2004-03-10波尔斯巴克有限公司

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
波尔斯巴克有限公司
Publication Date
2004-03-10
Estimated Expiration
Not applicable · inactive patent

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Abstract

In a method for detecting and identifying bioaerosol particles in the air, the bioaerosol particles in a particle stream are selected in an ATOFMS (aerosol time-of-flight mass spectrometer) by means of fluorescence techniques, and only the selected bioaerosol particles are ionized, for instance on the basis of MALDI (matrix-assisted laser desorption / ionization), after which the resulting ions are detected and the bioaerosol particles are identified. The selection of bioaerosol particles takes place by means of laser radiation, generated by a first laser device, of a wavelength which in specifice substances in bioaerosol particles effects a fluorescence, after which by means of a fluorescence detector the bioaerosol particles are selected and a second laser device is triggered to emit light of a wavelength which effects the ionization of the bioaerosol particles selected only by the fluorescence detector.
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Description

technical field

[0001] The present invention relates to a method and device for detecting and identifying bioaerosol particles in the air. Background technique

[0002] This method and the test device used can be obtained from the papers of M.A.Stowers et al.: Application of matrix-assisted laser desorption / ionization to on-line aerosol particle time-of-flight mass spectrometry (Application of matrix-assisted laser desorption / ionization to on-line aerosoltime-of-flight mass spectrometry), Rapid Commun. Mass Spectrom. 14, 829-833 (2000). The paper points out that there are two options for "quasi-real-time detection of bioaerosol particles", namely methods that utilize the inherent fluorescence of biomolecules, and methods that apply mass spectrometry to ions generated by laser desorption. Because bioaerosol particles form only a small fraction of the total aerosol particles in the air, well-known "single particle" fluorescence detectors can be used, indicating whether an aer...

Claims

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