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Method and device for detecting and identifying bio-aerosol particles in air

A bioaerosol and particle technology, applied in the direction of measuring devices, particle and sedimentation analysis, parts of particle separator tubes, etc., can solve unsatisfactory, insensitive bioaerosol particles and other problems

Inactive Publication Date: 2004-03-10
波尔斯巴克有限公司
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  • Application Information

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Problems solved by technology

However, in the described manner, it cannot be achieved satisfactorily, since the air mostly contains non-biological aerosol particles
Therefore, the ATOFMS detection and identification equipment used is not very sensitive to bioaerosol particles

Method used

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  • Method and device for detecting and identifying bio-aerosol particles in air
  • Method and device for detecting and identifying bio-aerosol particles in air

Examples

Experimental program
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Embodiment Construction

[0010] The ATOFMS shown in Figure 1 is shown in section, ie perpendicular to the flow of aerosol particles. The flow of aerosol particles essentially follows line 2 . Aerosol particles are drawn from the air into the ATOFMS, and the inlet part of the device is provided at high temperature with a coating originating from easily sublimable substances. For this purpose, it is known to use, for example, pyridinecarboxylic acid or sinapinic acid. The aerosol particles are then bundled by a pneumatic lens system, converging nozzle and skimmer. Figure 2 diagrammatically shows how the flow of aerosol particles enters the central space 7 of the ATOFMS from the nozzle 3 of the above-mentioned inlet section through the outlet 4 of 300 μm and the skimmer openings 5 ​​and 6 of 400 μm and 300 μm respectively. This space is continuously traversed by aerosol particles one after the other at a specific mutual distance. In this example, the velocity of aerosol particles entering the central ...

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Abstract

In a method for detecting and identifying bioaerosol particles in the air, the bioaerosol particles in a particle stream are selected in an ATOFMS (aerosol time-of-flight mass spectrometer) by means of fluorescence techniques, and only the selected bioaerosol particles are ionized, for instance on the basis of MALDI (matrix-assisted laser desorption / ionization), after which the resulting ions are detected and the bioaerosol particles are identified. The selection of bioaerosol particles takes place by means of laser radiation, generated by a first laser device, of a wavelength which in specifice substances in bioaerosol particles effects a fluorescence, after which by means of a fluorescence detector the bioaerosol particles are selected and a second laser device is triggered to emit light of a wavelength which effects the ionization of the bioaerosol particles selected only by the fluorescence detector.

Description

technical field [0001] The present invention relates to a method and device for detecting and identifying bioaerosol particles in the air. Background technique [0002] This method and the test device used can be obtained from the papers of M.A.Stowers et al.: Application of matrix-assisted laser desorption / ionization to on-line aerosol particle time-of-flight mass spectrometry (Application of matrix-assisted laser desorption / ionization to on-line aerosoltime-of-flight mass spectrometry), Rapid Commun. Mass Spectrom. 14, 829-833 (2000). The paper points out that there are two options for "quasi-real-time detection of bioaerosol particles", namely methods that utilize the inherent fluorescence of biomolecules, and methods that apply mass spectrometry to ions generated by laser desorption. Because bioaerosol particles form only a small fraction of the total aerosol particles in the air, well-known "single particle" fluorescence detectors can be used, indicating whether an aer...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/60G01N15/14G01N21/64G01N27/62G01N27/64H01J49/16H01J49/40
CPCH01J49/40H01J49/0445G01N15/14H01J49/164G01N15/1459G01N15/0205G01N15/01
Inventor 迈克尔·安东尼·斯托尔斯阿尔然·劳伦斯·威克黑兹约翰尼斯·科内利斯·马里亚·玛丽吉尼森查尔斯·伊丽莎·金茨
Owner 波尔斯巴克有限公司
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