Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for separating RNA from tissue sample

A technique for tissue samples and samples, applied in the field of biochemistry to achieve high activity

Inactive Publication Date: 2004-04-14
SHANGHAI CRIMINAL SCI TECH RES INST
View PDF0 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Many techniques exist for the purification of RNA from biological samples, but none for the reliable isolation of RNA from formalin-fixed, paraffin-embedded tissue samples

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0006] The present invention is a method for isolating RNA from a tissue sample. The method comprises the following steps: firstly dewaxing, the method of dewaxing uses xylene to wash the paraffin-embedded sample, and then utilizes an aqueous solution of a lower alcohol to rehydrate and Homogenization, the preferred lower alcohol is ethanol, the rehydration method is to gradually wash with the ethanol aqueous solution whose concentration gradually decreases, the sample is fully mixed with the alcohol solution and centrifuged, and then homogenized, the sample is dissolved in thiocyanic acid Carry out homogenization under the existence of guanidine chaotropic agent, the sample of homogenization is heated to 90-100 ℃, recovers RNA from solution then, the method used adopts the phenol chloroform extraction method in the prior art, then RNA can adopt Further purification by extraction, electrophoresis, chromatography, precipitation or other applicable techniques in the prior art.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention is a method for separating RNA form organism sample, the method includes: dewaxes at first, the invention uses dimethyl benzene to wash olefin and packages the sample, then uses lower alcohol liquid to carry on rehydration and homogeneity. the rehydration method is carrying on wash with alcohol solvent whose thickness is decreased gradually, the sample and the alcohol are blended fully and centrifugated, then carries on homogeneity again by rhodanic acid guanidine liquid. The samples are heated to 90í½100íµ, then reclaims RNA, the method is carbonic acid chloroform extracting method in current technology. Then the invention carries on extraction, cataphoresis, chromatography, depositing or other proper technology.

Description

Technical field: [0001] The invention relates to the field of biochemistry, in particular to the field of purifying RNA, DNA and protein in biological tissue samples, specifically a method for isolating RNA from tissue samples. Background technique: [0002] In the prior art, most pathological samples are often not prepared as frozen samples, but are more conventionally fixed in formalin and embedded in paraffin, so as to facilitate histological analysis and archival storage. In such fixed, embedded samples, protein expression levels can be measured using immunohistochemical staining, thereby semiquantitatively and indirectly measuring gene expression levels. Since paraffin-embedded samples are widely available, there is a need for a fast and reliable method for isolating nucleic acids, especially RNA, from samples. Many techniques exist for the purification of RNA from biological samples, but none exists for the reliable isolation of RNA from formalin-fixed, paraffin-embed...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C07H21/02
Inventor 曾立波
Owner SHANGHAI CRIMINAL SCI TECH RES INST
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com