Immune regulation and biological therapeutic function of benzo-isoselenazole derivatives
A technology of benzisoselenazole and derivatives is applied in the field of benzisoselenazole derivatives, which can solve the problems such as immunomodulation research and biological treatment applications that have not yet been reported, and achieve the effect of enhancing immune function.
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Embodiment 1
[0051] Example 1: Study on the Immunomodulatory Effect of Drugs on BALB / C Normal Mice
[0052] Animal grouping and dosing:
[0053] BALB / C mice were randomly divided into 4 groups, 20 animals in the experimental drug group, intraperitoneally injected with freshly prepared drugs, 10 mice at a dose of 25.0 mg / kg, and the other 10 mice at a dose of 12.5 mg / kg, once a day, continuously administered 7 days; 10 animals in the positive control group were intraperitoneally injected with the positive control drug ddp at a dose of 2.0 mg / kg, once a day for 7 consecutive days. In the control group, 10 rats were intraperitoneally injected with solvent, and the volume was adjusted according to body weight, which was the same as that of the drug group. Inject once a day for 7 consecutive days.
[0054] The mice in each of the above groups were given continuous administration, and were killed by necking on the 3rd day after drug withdrawal. The spleen was aseptically taken out, weighed wet...
Embodiment 2
[0060] Example 2: Immunomodulatory Effects of Drugs on IC2 of Common Grade 1 Albino Mice
[0061] Thirty-two IC2 mice with a weight of about 25 grams were randomly divided into 4 groups, 8 mice in each group. The first group was injected intraperitoneally with a dose of 25 mg / kg for 4 consecutive days; the second group was injected intraperitoneally with a dose of 12.5 mg / kg. mg / kg, for 4 consecutive days; the third group was injected intraperitoneally with DDP at a dose of 2 mg / kg; the fourth group was injected with the same volume of solvent intraperitoneally, for 4 consecutive days. On the fifth day, they were killed by necking, the spleen was taken, and the mononuclear cells were separated with lymphocyte separation medium to adjust the cell concentration to 2×10 6 / ml, as effector cells of NK cells. Take 3.6ml of mononuclear cell suspension, add 10 4 U / ml of IL 2 0.4ml, into a plastic culture bottle, after 48 hours of culture, absorb the culture medium and suspended c...
Embodiment 3
[0068] Example 3: Research on the bioregulatory effect of drugs on C57 tumor mice
[0069] (1) C57 tumor mice were injected intraperitoneally with EB (25 mg / kg), DDP and solvent respectively, and administered continuously for 7 days, and were killed by necking on the 10th day, and the spleen was taken out to weigh the spleen, and the mononuclear cells were isolated, and the proliferation of spleen cells was measured by MTT method Activity and NK / LAK cell activity, MTT method to measure NK cell activity, the method is the same as above. The results of spleen weight and spleen cell proliferation are shown in Table 3.
[0070] drug group
Positive Drug Panel (DDP)
blank control
Dose (mg / kg)
25
2
Splenocyte Proliferation (OD)
0.395
0.025
0.058
Spleen weight (g)
0.428
0.135
0.305
NK activity (%)
20.06
10.21
10.32
[0071] It can be seen from the table that the splenocy...
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