Method of determining latent heat of biological sample in freezing or melting process and its device

Abstract

The device consists of refrigerator, sample chamber, temperature transducer, auxiliary flat sheet, data collector and computer as thermocouple is connected to data collector connected with computer. The method is carried out as freezing or melting biological sample to be measured for generating temperature field in sample, collecting transient temperature information of temperature field along melting or freezing direction spac and to input information into computer for carrying on limited difference data processing, obtaining temperature gradient, heat conductivity, moving rate at two sides of solid and liquid boundary surface along freezing or melting direction of biological sample to be measured and utilizing energy continuous equation to calculate out latent heat of the biological sample to be measured.

Description

technical field [0001] The invention relates to a measuring method and device for measuring the latent heat of a biological sample to be measured based on the temperature information of the biological sample during the freezing or melting process. Background technique [0002] Low temperature biology is an interdisciplinary subject that has emerged and developed rapidly in recent years. With the deepening of research, its applications in various industries have attracted attention. In medicine, cryopreservation and transplantation of medical biomaterials is one of the most active fields in cryomedicine. Cryopreservation of medical biomaterials includes: cryopreservation of blood cells, hematopoietic cells, and germ cells; cryopreservation of skin, cornea, bone and cartilage, heart valves and large blood vessels, endocrine glands, and organs. In addition to cryopreservation, cryosurgery is another very important application of cryobiology in medicine. Cryosurgery is the use...

AI Technical Summary

Problems solved by technology

On the one hand, the instruments used in this method are very expensive and very cumbersome to operate

The most important thing is that due to the limitation of its principle, the method must adopt a sufficiently small cooling and heating rate, so it is impossible to measure the latent heat of phase change under different cooling and heating rates.

In addition, the latent heat measured by the DSC method is actually the sum of latent heat and sensible heat, but the sensible heat is relatively much smaller than the latent heat, which will inevitably introduce new measurement errors

At present, there is no method to measure the latent heat of phase change under different cooling and heating rates.

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