Low temperature method for conserving ture skin obtained from tissue engineering

A tissue engineering and cryopreservation technology, applied in the field of biomedicine, can solve problems such as the inability to meet long-term preservation

Inactive Publication Date: 2004-08-11
UNIV OF SHANGHAI FOR SCI & TECH +1
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  • Summary
  • Abstract
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  • Claims
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Problems solved by technology

As for the cryopreservation of tissue-engineered dermis with polyglycolic acid (PGA) fibers as scaffolds, the commercialized Dermagraft (Advanced Tissue Scince, La Jolla, Calif) is stored at -70°C, which cannot meet the requirements for long-term storage.

Method used

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  • Low temperature method for conserving ture skin obtained from tissue engineering
  • Low temperature method for conserving ture skin obtained from tissue engineering

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Embodiment Construction

[0015] Depend on figure 1 As shown, the cryopreservation process of tissue-engineered dermis is as follows: adding cryoprotective solution to the tissue-engineered dermis cultured for 9 days—pretreatment at 4°C for 15 minutes—programmed cooling—storage in liquid nitrogen for 24 hours— - rewarming - detection. Its specific implementation steps are as follows:

[0016] 1. Tissue-engineered dermis (1 cm) cultured for 9 days with uniform cell growth 2 ) Use surgical scissors to cut into (10×2×1.5) mm tissue strips in the ultra-clean bench, and place them in a sterile incubator for use;

[0017] 2. Preparation of temperature protection solution

[0018] Using DMEM culture medium (containing 10% calf serum) as a solvent, prepare a cryoprotective solution with a DMSO (dimethyl sulfoxide) concentration of 2.1 mol / L, and store it at 4°C for later use;

[0019] 3. Cryogenic protective agent treatment

[0020] Place the tissue-engineered dermis in a low-temperature protective agent ...

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Abstract

A low-temp storage method for the tissue-engineered skin includes such steps as sample treating, treating in DMSO solution at 4 deg.C for 15 min, putting it in low-temp storage bag, sealing, and lowering temp at 1 deg.C / min of speed. Its advantage is high survival rate of cells (72.8-78%).

Description

technical field [0001] The invention relates to a low-temperature preservation method for tissue-engineered dermis, which belongs to the field of biomedicine. Background technique [0002] Tissue engineered skin refers to the in vitro compound culture of epidermal cells or fibroblasts isolated from the body on a three-dimensional growth scaffold to form a regenerated dermis equivalent or skin equivalent. Among them, the equivalent of the dermis formed after the composite culture of fibroblasts and scaffold materials is the basis of skin tissue engineering. Tissue-engineered skin, as a skin wound repair material and a substitute for damaged skin, can allow patients with extensive and deep skin burns to undergo repair treatment and restore the loss of skin due to skin trauma in the case of insufficient autologous skin. Physiological function. Tissue-engineered skin not only covers the wound surface, protects the wound, anti-virus, sterilizes, and promotes the recovery and gr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/00A01N1/02A61L27/60
Inventor 王欣华泽钊杨光辉崔磊刘伟曹谊林
Owner UNIV OF SHANGHAI FOR SCI & TECH
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