Novel use of Brazil Hematine red
A technology of hematoxylin and disease, applied in the field of Brazilian hematoxylin in medicine, can solve problems such as unreported
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Embodiment 1
[0041] Take 0.5 kg of hematoxylin and use 80% ethanol which is 6 times its weight to reflux and extract once, each time for 1 hour. The extract was decompressed, concentrated at a temperature of 45°C, recovered ethanol, dried, and weighed. Add methanol to dissolve, stir into the polyamide column, and then use 4 times column volume of water and 4 times column volume of 90% ethanol to elute respectively, and collect the latter part of the eluate. The collected eluate was concentrated under reduced pressure at a temperature of 30° C., and dried under reduced pressure to obtain brazilianin. Measured by high performance liquid chromatography, the brazilian hematoxylin content is about 80%.
Embodiment 2
[0043] Get 0.5 kg of hematoxylin and use 15 times of the weight of the material to extract 2 times with 80% ethanol under reflux, each time for 3 hours. Concentrate the extract to recover ethanol, mix it with silica gel and pack it into a column, elute with 6 times column volume of ethanol-chloroform (20:80), and collect the eluate. The collected eluate was concentrated under reduced pressure at 60° C., and the precipitate was dried under normal pressure to obtain brazilian hematoxylin. The brazilian hematoxylin is determined by high performance liquid chromatography, and the brazilian hematoxylin content is about 90%.
Embodiment 3
[0045] Get 0.5 kg of hematoxylin and extract with 95% ethanol which is 10 times of its weight under reflux, at a reflux temperature of 80° C., and extract 3 times, each time for 1 hour. Concentrate the extract under reduced pressure at 50°C, recover ethanol, add water, extract with petroleum ether, and concentrate the extract under normal pressure to obtain brazilian hematoxylin. The brazilian hematoxylin is determined by high performance liquid chromatography, and the brazilian hematoxylin content is about 92%.
[0046] In the application of medicaments, brazilian hematoxylin is prepared into medicaments by conventional preparation process either alone or in a compound. The medicament can be various dosage forms that can be used clinically.
[0047] Determination of Brazilian hematoxylin, using high performance liquid chromatography to determine its content.
[0048] 1. Reagents and instruments
[0049] Waters high performance liquid chromatography, 515 pumps, 996 diode arra...
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