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Mineralized fibroin material and preparation method

A technology of silk protein and protein, applied in medical science, prosthesis, etc., can solve problems such as high cost, safety issues, complex collagen purification process, etc., and achieve good biocompatibility and bioactivity effects

Inactive Publication Date: 2005-01-12
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the complex purification process of the collagen used, the cost is high; because the currently used collagen is mainly derived from animals, there are certain safety issues; yet to be tested

Method used

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  • Mineralized fibroin material and preparation method
  • Mineralized fibroin material and preparation method
  • Mineralized fibroin material and preparation method

Examples

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Effect test

preparation example Construction

[0027] The preparation method of the mineralized silk protein material proposed by the present invention mainly comprises the following steps:

[0028] (1) Add a solution containing calcium ions dropwise in the silk protein solution, so that the mass percent concentration of silk protein in the solution is finally 0.3%-20%, and the molar concentration of calcium ions is finally 0.01-2mol / L. need constant stirring;

[0029] (2) Slowly add the aqueous solution containing phosphate ion while stirring in the solution of the above-mentioned first step, the mol ratio of the amount of phosphate ion added and the amount of calcium ion added is Ca: P=1~2:1 ;

[0030] (3) Slowly adjust the pH value to 7.4~13 while stirring in the solution of the above-mentioned second step,

[0031] (4) Stir the solution and let it stand. After the precipitate and supernatant are separated, remove the supernatant, centrifuge to separate the precipitate, wash repeatedly with deionized water to remove s...

Embodiment 1

[0033] 1) Add dropwise 500ml of Ca(OH) with a concentration of 0.25mol / L in the 500ml silk protein solution with a concentration of 0.9%. 2 After solution, continue to slowly add NaH with a concentration of 0.25mol / L 2 PO 4 Solution 300ml, mix evenly with a magnetic stirrer while adding dropwise.

[0034] 2) Stir the above system, let it stand, remove the supernatant, centrifuge to separate the precipitate, wash it repeatedly with deionized water to remove the salt and reach neutrality, put it into a lyophilizer to freeze dry, and then grind the dried product A dry powder was prepared.

Embodiment 2

[0036] 1) Add 25ml of CaCl with a concentration of 0.2mol / L to the 450ml silk protein solution with a concentration of 33%. 2 After the solution, continue to slowly drop Na with a concentration of 0.2mol / L 2 HPO 4 Solution 25ml, mix evenly with a magnetic stirrer while adding dropwise.

[0037] 2) Slowly add NaOH solution dropwise to the solution in the first step above while stirring until the pH value is stable at 7.4.

[0038] 3) Stir the above system, let it stand, remove the supernatant, centrifuge to separate the precipitate, wash it repeatedly with deionized water to remove the salt and reach neutrality, put it into a freeze dryer to freeze dry, and then grind the dried product A dry powder was prepared.

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PUM

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Abstract

A mineralized silk protein for inducing growth of bone is prepared through preparing water-soluble deglued silk protein, adding Ca ions contained solution to its solution while stirring, slowly adding PO4 ions contained solution, regulating pH value, stirring, laying aside, centrifugal separation-washing several times, and vacuum drying.

Description

technical field [0001] The invention relates to a mineralized silk protein material and a preparation method thereof, in particular to a mineralized silk protein material which can be used for bone repair and a preparation method thereof, and belongs to the field of biomedical materials. Background technique [0002] Many insects such as silkworms, tussahs, etc. and many species of spiders are able to produce large amounts of silk protein. In addition to being used as traditional textile raw materials, silk protein is being continuously developed and utilized in cosmetics, food, pharmaceuticals, chemicals, new materials and other fields. With the advancement of molecular biology and biotechnology, researchers are able to study silk proteins at the molecular level, and the economical and efficient production of silk proteins through transgenic methods has become the common goal of many scientists. At present, great progress has been made in transgenic research on silk and sp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/22
Inventor 崔福斋孔祥东张敏
Owner TSINGHUA UNIV
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