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Method for separating and preparing prodigiosin

A technology for prodigiosin and Serratia marcescens, which is applied in the direction of fermentation and the like, can solve the problems of large consumption of organic solvents, low product yield, many production links and the like, achieves fewer production links, simplified separation steps, The effect of improving product yield and production efficiency

Inactive Publication Date: 2005-01-12
EAST CHINA UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are many production links in this process, the consumption of organic solvent is large, and the yield of the product is low, generally only 50%.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Connect a resin column (φ60×300mm) to the outside of the 5L fermenter (with 2.5L fermentation broth inside), the amount of resin used is 8% of the volume of the fermentation broth, and a pump is used as the power to make the fermentation broth pass through the resin column, the bottom goes in and the top goes out, and passes through the resin The fermented liquid flows back into the tank, and the prodigiosin produced during the fermentation process is adsorbed on the resin, and the bacteria can return to the fermented liquid through the resin to continue its action. After 38 hours of fermentation, the fermentation ends, and at this time, the fermented liquid in the fermented liquid The prodigiosin content is already very low, and most of it is adsorbed to the resin.

[0026] The adsorbed saturated resin was washed with deionized water and methanol / water (1:1) to remove impurities, the amount used was 5 column volumes each, and then eluted with methanol. The methanol in ...

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Abstract

This invention discloses a method for preparing prodigiosin by separation utilizing big hole adsorption to adsorb prodigiosin directly in the fermented solution and realizing coupling of fermentation and separation to finish the process of concentration and initial impurities remove on the adsorption post. Then, according to the composition in the eluent, a suitable solvent system is applied to adsorb impurities instead of the products to get rather pure products.

Description

technical field [0001] The invention relates to a method for separating and preparing prodigiosin, in particular to a method for directly separating and preparing prodigiosin from a fermentation broth. Background technique [0002] Prodigiosin is a red pigment with a tripyrrole ring produced by Serratia marcescens during the fermentation process. Its structural formula is: [0003] Molecular weight: 324 [0004] After research, it has the biological activity of promoting the apoptosis of cancer cells, and is a candidate compound with the potential of anticancer drugs. Existing general preparation methods, such as (Montaner, B. and Pérez-Tomás, R., 2000.British Journal of Pharmacology 131,585-593.) The technology disclosed in the document is produced by Serratia marcescens by fermentation After the fermentation, the bacteria are obtained by centrifugation, and then extracted with methanol. After the bacteria are separated, the extract is concentrated, and finally separate...

Claims

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Application Information

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IPC IPC(8): C12P17/16
Inventor 魏东芝王学东沈亚领
Owner EAST CHINA UNIV OF SCI & TECH
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