Conservation and breeding method for rice photosensitive and temperature-sensitive sterile line

A temperature-sensitive sterile line and temperature-sensitive nuclear technology is applied in botany equipment and methods, horticultural methods, seed and rhizome treatment, etc., which can solve problems such as genetic drift and loss of practical value, and achieve improved genetic stability and maintenance highly consistent effect

Inactive Publication Date: 2005-01-19
HUAZHONG AGRI UNIV
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Problems solved by technology

[0009] The purpose of the present invention is to overcome the defects in the prior art, and propose a method for preserving and propagating rice light- and temperature-sensitive genic male sterile lines, so as to solve the problems of existing rice light- and temperature-sensitive genic male sterile lines due to the increase of breeding generations. The genetic drift of the sterility starting point temperature leads to the loss of practical value of the sterile line, and ensures that the obt

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  • Conservation and breeding method for rice photosensitive and temperature-sensitive sterile line

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Embodiment 1

[0029] The technical key of the present invention is to obtain the homozygous single plant of the light- and temperature-sensitive genic male sterile line, vegetative body preservation and core seed propagation through strict light-temperature identification, and its specific implementation scheme is as follows:

[0030] 1. Select rice light-temperature interactive CMS Peiai 64S, photosensitive CMS W9593S and M201S, temperature-sensitive CMS M102S and M103S with practical production value, and plant each CMS in seedling trays 200 seedlings were transplanted at the 5-leaf stage into a nutrient pot with a diameter of 30 cm, and 5 seedlings were planted in each pot. When most of the plants develop and enter the secondary branch differentiation stage of young ears, the nutrient pots are moved into the artificial climate chamber. The light and temperature conditions of the artificial climate chamber are: the daily average temperature is 23.5°C, the day-night variation is 19-27°C, t...

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Abstract

The invention discloses a conservation and breeding method for rice photosensitive and temperature-sensitive sterile line, wherein the sterile lines are subject to twice linear light cryogenic temperature determination, and strains with low sterility initial point temperature and stable genetic homozygosity are selected for trophosome permanent conservation, and kern seeds are breeded from the expansion of the conserved trophosomes, thus realizing the mass propagation of sterility seeds for production of hybrid seeds.

Description

technical field [0001] The invention belongs to the technical field of new crop variety selection and breeding, and in particular relates to a method for preserving and propagating rice light- and temperature-sensitive genetic male sterile lines. Background technique [0002] Academician Yuan Longping once published a paper entitled "Purification and Original Seed Production of Rice Light- and Temperature-Sensitive CMS Lines" on pages 1-3 of Issue 6 of "Hybrid Rice" in Chinese and English in 1994. It is believed that "the starting temperature leading to male sterility must be relatively low, which is the most important technical index for breeding practical light- and temperature-sensitive male sterile lines of rice". This argument was proved to be correct by subsequent breeding and production practices. This paper also believes that: the fertility of rice light- and temperature-sensitive CMS has the characteristics of fluctuations with temperature c...

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Application Information

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IPC IPC(8): A01C1/00A01G7/00
Inventor 牟同敏李春海
Owner HUAZHONG AGRI UNIV
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