Conservation and breeding method for rice photosensitive and temperature-sensitive sterile line

Abstract

The invention discloses a conservation and breeding method for rice photosensitive and temperature-sensitive sterile line, wherein the sterile lines are subject to twice linear light cryogenic temperature determination, and strains with low sterility initial point temperature and stable genetic homozygosity are selected for trophosome permanent conservation, and kern seeds are breeded from the expansion of the conserved trophosomes, thus realizing the mass propagation of sterility seeds for production of hybrid seeds.

Description

technical field [0001] The invention belongs to the technical field of new crop variety selection and breeding, and in particular relates to a method for preserving and propagating rice light- and temperature-sensitive genetic male sterile lines. Background technique [0002] Academician Yuan Longping once published a paper entitled "Purification and Original Seed Production of Rice Light- and Temperature-Sensitive CMS Lines" on pages 1-3 of Issue 6 of "Hybrid Rice" in Chinese and English in 1994. It is believed that "the starting temperature leading to male sterility must be relatively low, which is the most important technical index for breeding practical light- and temperature-sensitive male sterile lines of rice". This argument was proved to be correct by subsequent breeding and production practices. This paper also believes that: the fertility of rice light- and temperature-sensitive CMS has the characteristics of fluctuations with temperature c...

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Problems solved by technology

[0009] The purpose of the present invention is to overcome the defects in the prior art, and propose a method for preserving and propagating rice light- and temperature-sensitive genic male sterile lines, so as to solve the problems of existing rice light- and temperature-sensitive genic male sterile lines due to the increase of breeding generations. The genetic drift of the sterility starting point temperature leads to the loss of practical value of the sterile line, and ensures that the obtained rice photo- and temperature-sensitive genic male sterile line with stable fertility comes from a homozygous rice photo- and temperature-sensitive genic male sterile line Individuals, the long-term preservation of the sterile line can be achieved through the preservation of rice thorns. At the same time, through the designed reproduction and identification procedures, the reproductive efficiency of the rice light- and temperature-sensitive genic male sterile line can be greatly improved.

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