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Method for rapid analysing power composition of soybean phospholipid

A technology for rapid analysis of soybean lecithin, which is applied in the field of low-cost analysis of powder soybean lecithin components, is fast and simple, and can solve the problems of pollution, high price, waste of the environment, etc.

Inactive Publication Date: 2005-05-04
李卫
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method requires the use of pure standards, and these pure standards for analysis and detection are extremely expensive, unstable, difficult to store, and almost disposable
Secondly, it takes a long time to analyze a thin-layer chromatography plate of 20cm×20cm, and the amount of developer used is too large, causing a lot of waste and environmental pollution

Method used

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Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment approach 2

[0017] Quantitative analysis of powdered soybean lecithin components: Accurately weigh 30 mg powdered soybean lecithin and dissolve it in chloroform to prepare a 1% powdered soybean lecithin chloroform solution. Draw 10 μl of this solution with a micro-syringe, and apply the sample at a place about 0.5-1 cm away from the bottom of the thin-layer chromatography plate. Put the thin-layer chromatography plate after sampling into a beaker filled with developer, and seal the upper part of the beaker with a plastic film to prevent the solvent from volatilizing. When the developer rises to 4 / 5 of the height of the thin-layer chromatography plate, take out the thin-layer chromatography plate. Place the above-mentioned thin-layer chromatography plate at room temperature for a few minutes, and after the solvent evaporates, use a chromogenic agent for combined color development, and use the phosphorus determination method to calculate the content of each phospholipid.

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PUM

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Abstract

This invention is a kind of method that can quickly analyze the powder soy bean phosphatide components. It uses 2.5cm *7.5cm silica gel that made from glass slide as thin-layer chromatography lamina and a high efficiency developing agent to make the powder soy bean phosphatide components to unfold and separate quickly on the 2.5cm *7.5cm thin-layer chromatography lamina. Then we use several color-developing agents to assembly develop and ascertain the components and volume of the powder soy bean phosphatide components, and it needn't dear standard substance. The time needed is only 1 / 5 of the 20cm *20cm thin-layer chromatography lamina and the dosage of the color-developing agent is only 5-10ml.

Description

technical field [0001] The invention relates to the field of product analysis and detection, in particular to improving and innovating an existing analysis method for powdered soybean lecithin components, so as to achieve the purpose of analyzing powdered soybean lecithin components quickly, easily and at low cost. Background technique [0002] The conventional phospholipid method can only analyze the total phospholipid content in the powdered soybean phospholipid sample, but cannot determine the various phospholipid components (lecithin, cephalin, inositol phospholipid) and their content in the powdered soybean phospholipid sample. [0003] Foreign countries usually use high performance liquid chromatography or nuclear magnetic resonance 31 The P-NMR method analyzes powdered soybean phospholipids, but these two instruments are expensive and not available in ordinary laboratories. Therefore, analyzing the components of powdered soybean phospholipids has always been a diffic...

Claims

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Application Information

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IPC IPC(8): G01N30/90G01N30/94G01N30/95
Inventor 李卫
Owner 李卫