Cell quantitative analysis method based on micro fluid control chip

A microfluidic chip and qualitative analysis technology, which is applied in the direction of microbial measurement/inspection, analytical materials, biochemical equipment and methods, etc., can solve the problems not mentioned in the application of cell classification, etc., and achieve simple instruments, flexible operation, and small size. The effect of method interference

Inactive Publication Date: 2005-05-18
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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Problems solved by technology

None of the above methods for measuring electrophoretic mobility mention its use in cell sorting

Method used

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  • Cell quantitative analysis method based on micro fluid control chip
  • Cell quantitative analysis method based on micro fluid control chip
  • Cell quantitative analysis method based on micro fluid control chip

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Embodiment Construction

[0025] Take 13uL sample and add it to the buffer pool. Due to the height difference of the liquid level, the liquid continuously fills each channel, then add 13uL buffer solution to the waste liquid pool, carefully adjust the liquid level height of each liquid pool, so that the particles or cells Staying still in the channel eliminates the effects of static pressure differences. Then a positive voltage is connected to the buffer pool, the waste liquid pool is grounded, and the particles or cells migrate from the positive pole to the negative pole. Microscope imaging, CCD recording. The CCD recording result is 25 frames per second, select a single particle to record its initial moment, save the picture at this moment, see figure 1 , record its starting position. Find the end moment and end position of the same particle in a frame-forward manner, so as to calculate the particle velocity. The number of recorded particles is more than 30 under each electric field.

[0026] i...

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Abstract

The qualitative cell analysis method based on microfluid control chip features the single channel microfluid control chip as platform, the sample pond and the buffering pond on two ends of the channel and microscope coupled in any position to the channel. The analysis process includes: feeding cell liquid into the buffering pond and buffering liquid into the waste liquid pond and regulating the liquid surface height in the buffering pond to make cells inside the visual field still; applied voltage across the buffering pond and the waste liquid pond, recording with CCD on the microscope the directional shift speed of each cell and calculating the electrophoresis mobility of each cell; drawing the cell number- electrophoresis mobility curve of the tested cells; and comparing the curve with standard curves to obtain the qualitative cell analysis result. The method of the present invention has less interference and no need of separation or sorting of cell groups.

Description

Technical field: [0001] The invention relates to a cell qualitative analysis technology, and in particular provides a cell qualitative analysis method based on a microfluidic chip and using electrophoretic mobility as a parameter. Background technique: [0002] Cell sorting is one of the means to understand complex cell populations. Different from cell sorting and isolation, it can achieve preliminary understanding of cell populations without going through the isolation process. All existing commercialized instruments need to sort or separate cells to complete cell classification, and there is no sorter that does not require cell separation. [0003] Cell electrophoretic mobility is one of the parameters that characterize the cell surface charge [Biophysics edited by Lin Kechun, Beijing: People's Education Press, 1981; 267-282], and its commonly used measurement methods include cell electrophoresis [Hashimoto, N., Fujita, s., Yokoyama, T., Ozawa, Y., Kingstsu, I., Kurosaka...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/04G01N27/447G01N33/50
Inventor 盖宏伟於林芬马银法林炳承
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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