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Self-coagulating producing hydrogen bacteria and its screening process

A screening method and self-flocculation technology, applied in the directions of bacteria, botanical equipment and methods, biochemical equipment and methods, etc., can solve the problems of increasing operating costs, low reaction system, limiting hydrogen production efficiency, etc., to reduce operating costs, The effect of high hydrogen production capacity and strong self-flocculation capacity

Inactive Publication Date: 2005-06-08
HARBIN INST OF TECH
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Problems solved by technology

Because the growth conditions of the hydrogen production reactor are different from those of the static culture, especially the pH of the reaction system during the acidogenic fermentation process is low, so although the high-efficiency fermentative bacteria isolated so far have high hydrogen production capacity, they cannot It often cannot achieve its optimal hydrogen production and growth conditions. High-efficiency hydrogen-producing bacteria cannot become the dominant population in the microbial community. They can only be immobilized, which limits their hydrogen production efficiency and increases operating costs.

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  • Self-coagulating producing hydrogen bacteria and its screening process
  • Self-coagulating producing hydrogen bacteria and its screening process

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specific Embodiment approach 1

[0009] Specific embodiment one: Harbin ethanologen YUAN-3 (Ethanologenbacterium Harbin YUAN-3) of this embodiment is selected according to the following steps:

[0010] 1. Isolation, purification and screening of bacterial strains: the improved bacterial isolation medium is used to isolate hydrogen-producing bacteria, and the specific steps are as follows: a. Take 1-2 mL of hydrogen-producing fermentation active sewage from the continuous flow biological hydrogen production reactor CSTR Sludge, put it into a Erlenmeyer flask filled with nitrogen, add several glass beads, shake on the shaker for 1 hour, break up the bacteria micelles in the sludge, and dilute to 10 with sterile water. -1 ~10 -11 ml; b. Inoculate 0.2-0.5ml of the strain into 10ml of solid medium to make a rolling tube and culture for 7-10 days; c. Pick a single colony and transfer it into the liquid medium to observe whether there are floc particles produced. Those with floc formation are self-flocculation bact...

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Abstract

A self-flocculating hydrogen-generating bacterium, Harbin ethanolgenic bacterium YUAN-3 (CGMCC No.1152) is disclosed, which is an obligate anaerobic bacillus with the metabolic characteristic of ethanol fermenting. Its maximal specific hydrogen generating rate is 22.6 mmlH2 / g drycell.h. It can become the depositive flocculated particles in the hydrogen generating bioreactor. The hydrogen can be continuously generated without carrier and filler.

Description

Technical field: [0001] The invention relates to a fermentative hydrogen-producing bacterium and a screening method thereof, in particular to a new species of self-flocculation fermentative hydrogen-producing bacterium Harbin YUAN-3 (Ethanologenbacterium Harbin YUAN-3) and a screening method thereof. Background technique: [0002] Biological hydrogen production technology is one of the important means to solve the energy crisis and environmental pollution problems. Due to its dual efficacy and sustainability, it has been increasingly valued by countries all over the world. Improving the efficiency of hydrogen production is an urgent problem to be solved in the industrialization of biohydrogen production technology. Screening efficient hydrogen-producing bacteria is an effective way to accelerate the industrialization of biological hydrogen production. In order to obtain efficient hydrogen-producing fermentation bacteria, foreign researchers isolated a large number of hydrog...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N15/31
Inventor 邢德峰任南琪李秋波
Owner HARBIN INST OF TECH
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