Self-coagulating producing hydrogen bacteria and its screening process
A screening method and self-flocculation technology, applied in the directions of bacteria, botanical equipment and methods, biochemical equipment and methods, etc., can solve the problems of increasing operating costs, low reaction system, limiting hydrogen production efficiency, etc., to reduce operating costs, The effect of high hydrogen production capacity and strong self-flocculation capacity
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[0009] Specific embodiment 1: The Ethanologenbacterium Harbin YUAN-3 (Ethanologenbacterium Harbin YUAN-3) of this embodiment is selected and bred according to the following steps:
[0010] 1. Isolation, purification and screening of strains: The improved bacterial separation medium is used to separate hydrogen-producing bacteria. The specific steps are as follows: a. Take out 1 to 2 mL of hydrogen-producing fermentation activated sewage from the continuous flow biological hydrogen production reactor CSTR Put the sludge into a triangular flask filled with nitrogen, add a few glass beads, shake on a shaker for 1 hour, break up the bacterial micelles in the sludge, and dilute to 10 with sterile water -1 ~10 -11 ml; b. Inoculate 0.2~0.5ml strains in 10ml solid medium, make a rolling tube, and culture for 7~10d; c. Pick a single colony and transfer it to the liquid medium to observe whether there are floccules. Flocs formed are self-flocculating bacteria; d. Repeat the above operation ...
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