Self-coagulating producing hydrogen bacteria and its screening process

A screening method and self-flocculation technology, applied in the directions of bacteria, botanical equipment and methods, biochemical equipment and methods, etc., can solve the problems of increasing operating costs, low reaction system, limiting hydrogen production efficiency, etc., to reduce operating costs, The effect of high hydrogen production capacity and strong self-flocculation capacity

Inactive Publication Date: 2005-06-08
HARBIN INST OF TECH
View PDF0 Cites 11 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because the growth conditions of the hydrogen production reactor are different from those of the static culture, especially the pH of the reaction system during the acidogenic fermentation process is low, so although the high-efficiency fermentative bacteria isolated so far have high hydrogen production capacity

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Self-coagulating producing hydrogen bacteria and its screening process
  • Self-coagulating producing hydrogen bacteria and its screening process

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0009] Specific embodiment 1: The Ethanologenbacterium Harbin YUAN-3 (Ethanologenbacterium Harbin YUAN-3) of this embodiment is selected and bred according to the following steps:

[0010] 1. Isolation, purification and screening of strains: The improved bacterial separation medium is used to separate hydrogen-producing bacteria. The specific steps are as follows: a. Take out 1 to 2 mL of hydrogen-producing fermentation activated sewage from the continuous flow biological hydrogen production reactor CSTR Put the sludge into a triangular flask filled with nitrogen, add a few glass beads, shake on a shaker for 1 hour, break up the bacterial micelles in the sludge, and dilute to 10 with sterile water -1 ~10 -11 ml; b. Inoculate 0.2~0.5ml strains in 10ml solid medium, make a rolling tube, and culture for 7~10d; c. Pick a single colony and transfer it to the liquid medium to observe whether there are floccules. Flocs formed are self-flocculating bacteria; d. Repeat the above operation ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

A self-flocculating hydrogen-generating bacterium, Harbin ethanolgenic bacterium YUAN-3 (CGMCC No.1152) is disclosed, which is an obligate anaerobic bacillus with the metabolic characteristic of ethanol fermenting. Its maximal specific hydrogen generating rate is 22.6 mmlH2/g drycell.h. It can become the depositive flocculated particles in the hydrogen generating bioreactor. The hydrogen can be continuously generated without carrier and filler.

Description

Technical field: [0001] The invention relates to a fermentative hydrogen-producing bacterium and a screening method thereof, in particular to a new species of self-flocculation fermentative hydrogen-producing bacterium Harbin YUAN-3 (Ethanologenbacterium Harbin YUAN-3) and a screening method thereof. Background technique: [0002] Biological hydrogen production technology is one of the important means to solve the energy crisis and environmental pollution problems. Due to its dual efficacy and sustainability, it has been increasingly valued by countries all over the world. Improving the efficiency of hydrogen production is an urgent problem to be solved in the industrialization of biohydrogen production technology. Screening efficient hydrogen-producing bacteria is an effective way to accelerate the industrialization of biological hydrogen production. In order to obtain efficient hydrogen-producing fermentation bacteria, foreign researchers isolated a large number of hydrog...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N1/20C12N15/31
Inventor 邢德峰任南琪李秋波
Owner HARBIN INST OF TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap