Populus diversifolia tissue culture quick breeding technology

A technology of tissue culture and Populus euphratica, which is applied in horticulture, botanical equipment and methods, gardening tools/equipment, etc., can solve the problems of weak strength and small number of seedlings, and achieve the effect that the method is feasible and meets the needs of afforestation

Inactive Publication Date: 2005-08-03
上海光兆植物速生技术有限公司
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AI Technical Summary

Problems solved by technology

Disadvantages: too few seedlings
But to quickly solve the problem of Populus euphratica seedlings, the strength is weak

Method used

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  • Populus diversifolia tissue culture quick breeding technology

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Embodiment Construction

[0012] The Populus euphratica tissue culture rapid propagation technology of the present invention includes: explant material collection and aseptic treatment, inoculation and differentiation culture, rooting culture, seedling training and transplanting and other technical processes.

[0013] Among them, explant materials and methods: material collection and aseptic treatment: in fine weather from April to May, excellent mother plants of Populus euphratica were selected, and young shoots that were born in the same year without diseases and insect pests were cut as explants. Rinse with clean water for 1 hour, then sterilize with 5-10% hydrogen peroxide for 10-15 minutes, rinse with sterile water for 4-5 times, take out and put sterile filter paper to absorb the water.

[0014] Inoculation and differentiation culture: Cut the sterilized shoots with a sharp scalpel into 1cm-long segments with terminal buds and lateral buds on the sterile operating table, and inoculate them in: MS+...

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Abstract

The present invention relates to fast populus diversifolia breeding technology, and fast tissue culture method for fast breeding populus diversifolia and the formula of culture medium. The fast tissue culture technology includes selecting explant and bacteria-free treatment, inoculating and differentiating culture, rooting, hardening off and transplanting. The explant is healthy tender twig of selected populus diversifolia mother plant. The method of the present invention is feasible and is suitable for culturing great amount of populus diversifolia seedlings.

Description

technical field [0001] The populus euphratica tissue culture rapid propagation technology of the present invention relates to a populus euphratica tissue culture rapid propagation method and a culture medium formula. In order to quickly solve the seedlings of Populus euphratica. Background technique [0002] Populus euphratica is a large tree growing in desert areas, belonging to the Salicaceae family. It has good salt-alkali tolerance, drought resistance and cold resistance. However, due to various reasons, the number of Populus euphratica has been reduced, and it has been listed as a national third-class endangered plant list. The Chinese government attaches great importance to changing the arid and desert environment in Northwest China. As an excellent plant for improving the environment, Populus euphratica has the following conditions: first, it has strong adaptability and is a local traditional tree species; second, it is the most effective, and as an arbor, Populus ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 苏敏宋学孟庞瑞华章振华王凌健董举文何燕红王涛陈权苏峰
Owner 上海光兆植物速生技术有限公司
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