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Wireworm-killing biologic bacterial agent, its preparation and application

A biological inoculant and nematicide technology, applied in the directions of botanical equipment and methods, biocide, application, etc., can solve the problem that the action of Verticillium serrata has not yet been reported, and achieve the effect of good application and development prospects.

Inactive Publication Date: 2005-09-07
YUNNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, Verticillium lecanii and Prokonia chlamydoides have been used in the development of bio-insecticides, but there is no report about the effect of Verticillium canaliculus on insects and nematodes.

Method used

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  • Wireworm-killing biologic bacterial agent, its preparation and application
  • Wireworm-killing biologic bacterial agent, its preparation and application
  • Wireworm-killing biologic bacterial agent, its preparation and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] The YMF1.00112 strain was inoculated on a PDA slant, and cultured at 20-25°C for 8 days to obtain a test tube species.

[0048]Inoculate the test tube seed into 250ml Erlenmeyer flask liquid medium, each bottle contains 60ml, the liquid medium formula is: 10g glucose, 1g ammonium sulfate, 0.5g magnesium sulfate, 0.015g ferrous sulfate, 1000ml potato extract juice, pH6.5. Each 250ml Erlenmeyer flask was filled with 60ml of culture solution, sterilized at 121°C for 20 minutes, then inserted into the test tube, cultured on a shaker at 26°C and 200rpm for 6 days, then filtered the bacterial solution, and concentrated it with an ultrafiltration membrane with a pore size of 5KDa. After dialysis treatment, the concentrate was purified with a cation exchange column, and the breakthrough peak and elution peak were collected separately. The biological activity measurement showed that most of the protease activity was in the elution peak, and the combined elution peak was further p...

Embodiment 2

[0050] Basically the same as Example 1, the difference is: the formula of the liquid culture medium is 8g of glucose; 1.5g of ammonium sulfate; 0.6g of magnesium sulfate; 0.01g of ferrous sulfate.

Embodiment 3

[0052] Basically the same as Example 1, the difference is: the formula of liquid culture medium is: 15g glucose; 0.8g ammonium sulfate; 0.4g magnesium sulfate; 0.02g ferrous sulfate.

[0053] Use effect of the present invention sees Table 1 and Table 2 for details.

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PUM

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Abstract

The present invention relates to a process for preparing and application of bionematocide and belongs to technique domain of biological pesticide. Product of the invention can be gained from conventional method that the produced strain is cultivated in test tube, enlarged cultivated in liquid and extracts active constituents of nematocide, whose produced strain is Lecanicillium psalliotae YMF1.00112, with conservation number CGMCC No.1312. The formulation of enlarged liquid base in the invention is, dextrose 10g, ammonia sulfate 1g, magnesium sulfate 1g, ferrous sulfate 0.015g, leachate of potato 1000mg, pH6.5. the invention can poison pine wood nematode and Panagrellus redivivus and be used as bio-prevention to plant parasitic nematode. The product has the advantage of high poison effect, safety to human body and environment protection, etc.

Description

Technical field: [0001] The invention relates to a nematicide biological bacterial agent and its preparation method and application, belonging to the technical field of biological pesticides. Background technique: [0002] Plant nematodes are a common plant disease worldwide. There are more than 70 known species of root-knot nematodes alone, which harm more than 3,000 plants and cause huge losses every year. According to the statistics of the Food and Agriculture Organization of the United Nations, the annual global losses caused by nematodes are as high as more than 100 billion US dollars. In 2001, the losses caused by nematode damage to crops in my country were as high as 23.3 billion yuan. Pine xylophilus is known as smokeless forest fires, which have seriously occurred in some areas such as Jiangsu, Zhejiang, Guangdong, Shandong, Anhui, Hubei, Shanghai, Taiwan, Hong Kong, etc., and have a tendency to spread to the whole country. What's more serious is that root-knot nem...

Claims

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Application Information

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IPC IPC(8): A01N63/04C12N1/14
Inventor 张克勤杨金奎周薇
Owner YUNNAN UNIV
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