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Cofocal microscope, fluorescence measuring method and polarized light measuring metod using cofocal microscope

A confocal and microscope technology, used in microscopes, fluorescence/phosphorescence, measurement devices, etc., can solve the problem of difficult to observe fluorescence in real time, and achieve the effect of improving resolution and reducing intermodulation interference.

Inactive Publication Date: 2005-11-02
JAPAN SCI & TECH CORP
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

Compared with the single focus of the multiple confocal microscope of conventional example 1, although the time required for scanning is shortened, scanning is necessary to observe a wide area, and it is difficult to observe fluorescence, etc. in real time

Method used

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  • Cofocal microscope, fluorescence measuring method and polarized light measuring metod using cofocal microscope
  • Cofocal microscope, fluorescence measuring method and polarized light measuring metod using cofocal microscope
  • Cofocal microscope, fluorescence measuring method and polarized light measuring metod using cofocal microscope

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Embodiment Construction

[0056] Embodiments of the present invention will be described in detail below with reference to the drawings.

[0057] First, the first embodiment of the confocal microscope using liquid crystal of the present invention will be described. figure 1 It is a schematic diagram showing the structure of a confocal microscope using a liquid crystal according to the first embodiment of the present invention. The confocal microscope 1 using liquid crystal has: an illumination optical system 10; an incident optical system 20 including a matrix type liquid crystal device and forming multiple focuses on the object to be observed; a detection optical system 30 for detecting reflected light from the object to be observed ; a control system 50 for controlling the image data from the matrix liquid crystal device and the detection optical system; and a stage 3 for placing the observed object 2 .

[0058] The illumination optical system 10 is composed of an illumination light source 11 , a co...

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Abstract

The present invention relates to a confocal microscope and the measuring methods of fluorescence and the polarized light using the same, and said confocal microscope is provided with the inlet optical part ( 10, 10 ') to let the polarized light from an illuminating light source ( 11 ) onto an object to be observed ( 2 ) via a matrix type liquid crystal device ( 22 ) provided with a microlens array ( 21 ) on its top part, and an objective lens ( 23 ), the light detecting part ( 30, 30 ') to detect the reflected or the fluorescent light from the object to be observed, and the liquid crystal control subpart ( 52 ) to control a liquid crystal device ( 22 ), and it transmits the light passing through said microlens array ( 21 ) from each microlens to each pixel ( 22 a) of the liquid crystal device ( 22 ), and makes a plurality of foci ( 24 ) on the object to be observed ( 2 ) by the objective lens ( 23 ), as well as controls polarization directions of the lights transmitted through each pixel of the liquid crystal device ( 22 ) using the liquid crystal control subpart ( 52 ) so that they are made mutually orthogonal.

Description

technical field [0001] The present invention relates to a confocal microscope used for observing biological tissue or fluorescence emitted by biological tissue, and relates to a confocal microscope with high sensitivity, good resolution in the transverse direction and depth direction, and capable of dynamic observation in a wide range. Confocal microscopy of liquid crystals, as well as fluorescence measurement methods for microarray substrates based on confocal microscopy using liquid crystals and polarized light measurement methods based on confocal microscopy using liquid crystals. Background technique [0002] In the past, in the research field of life sciences, confocal microscopes have been used to observe the fluorescence emission of biological tissues or biological tissue samples added with fluorescent reagents. Since the confocal microscope has high resolution in the depth direction, it is mainly used for three-dimensional observation of biological samples and the li...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/21G01N21/64G02B21/00
CPCG01N21/6458G01N21/21G02B21/0068G01N21/6452G02B21/0076G01N2021/6478G02B21/0024G01N21/6445G01N2201/0675G01N2021/6471G02B5/3016
Inventor 林照刚前川克广柴田隆行
Owner JAPAN SCI & TECH CORP
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