Cofocal microscope, fluorescence measuring method and polarized light measuring metod using cofocal microscope

A confocal and microscope technology, used in microscopes, fluorescence/phosphorescence, measurement devices, etc., can solve the problem of difficult to observe fluorescence in real time, and achieve the effect of improving resolution and reducing intermodulation interference.
CN1692296AInactive Publication Date: 2005-11-02JAPAN SCI & TECH CORP
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
JAPAN SCI & TECH CORP
Publication Date
2005-11-02
Estimated Expiration
Not applicable · inactive patent

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Abstract

The present invention relates to a confocal microscope and the measuring methods of fluorescence and the polarized light using the same, and said confocal microscope is provided with the inlet optical part ( 10, 10 ') to let the polarized light from an illuminating light source ( 11 ) onto an object to be observed ( 2 ) via a matrix type liquid crystal device ( 22 ) provided with a microlens array ( 21 ) on its top part, and an objective lens ( 23 ), the light detecting part ( 30, 30 ') to detect the reflected or the fluorescent light from the object to be observed, and the liquid crystal control subpart ( 52 ) to control a liquid crystal device ( 22 ), and it transmits the light passing through said microlens array ( 21 ) from each microlens to each pixel ( 22 a) of the liquid crystal device ( 22 ), and makes a plurality of foci ( 24 ) on the object to be observed ( 2 ) by the objective lens ( 23 ), as well as controls polarization directions of the lights transmitted through each pixel of the liquid crystal device ( 22 ) using the liquid crystal control subpart ( 52 ) so that they are made mutually orthogonal.
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Description

technical field

[0001] The present invention relates to a confocal microscope used for observing biological tissue or fluorescence emitted by biological tissue, and relates to a confocal microscope with high sensitivity, good resolution in the transverse direction and depth direction, and capable of dynamic observation in a wide range. Confocal microscopy of liquid crystals, as well as fluorescence measurement methods for microarray substrates based on confocal microscopy using liquid crystals and polarized light measurement methods based on confocal microscopy using liquid crystals. Background technique

[0002] In the past, in the research field of life sciences, confocal microscopes have been used to observe the fluorescence emission of biological tissues or biological tissue samples added with fluorescent reagents. Since the confocal microscope has high resolution in the depth direction, it is mainly used for three-dimensional observation of biological samples and the li...

Claims

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