Strain specificity PCR detection method of heredity modified corn strain MON863

A technology of MON863 and detection method, applied in the field of bioengineering

Inactive Publication Date: 2006-01-25
SHANGHAI JIAO TONG UNIV
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Problems solved by technology

So far, there have been reports of line-specific detection methods for a considerable number of transgenic plant lines, such as Roundup Read

Method used

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Experimental program
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Effect test

Embodiment 1

[0023] 1. Experimental materials

[0024] 1. Plant material

[0025]Genetically improved corn strains: MON863 corn, MON810 corn, Bt11 corn, Bt176 corn, TC1507 corn, 6A21 corn, etc.

[0026] conventional corn

[0027] Other transgenic plants: Roundup Ready soybeans, GT73 rapeseed, MON531 cotton, "Huafan No. 1" tomato, etc.

[0028] 2. Enzymes and reagents

[0029] The Plant Genome DNA Extraction Kit is a food DNA extraction kit jointly developed by Shanghai Academy of Agricultural Sciences and Shanghai Entry-Exit Inspection and Quarantine Bureau.

[0030] dNTPs, Taq DNA polymerase and its buffer, and DL2000 Marker were purchased from Dalian Bao Biological Engineering Co., Ltd. Random primers, TaqMan probes and primers were synthesized by Shanghai Boya Bioengineering Co., Ltd.

[0031] Other biochemical reagents are imported subpackages or domestic analytical pure.

[0032] 3. Experimental equipment

[0033] DY-501 Nucleic Acid Electrophoresis Apparatus (Shanghai Precisio...

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Abstract

The invention relates to strain specificity PCR testing method of improved corn strain MON863 that includes 5'end strain specificity testing method and 3'end strain specificity testing method. The process includes: analyzing external inserting carrier neighbor gene sequence of MON863 corn, designing primer of strain specificity PCR and ration PCR, and probe; building and optimizing strain specificity and ration PCR system; verifying the strain specificity and ration PCR testing method to build the testing platform of strain specificity PCR of improved corn strain MON863.

Description

technical field [0001] The invention relates to a detection method in the technical field of bioengineering. Specifically, it relates to a line-specific PCR detection method for a genetically improved maize line MON863. Background technique [0002] With the rapid development of modern biotechnology, genetic engineering technology has been widely used in the field of agricultural production. Hundreds of genetically modified crops have been widely planted around the world, providing human beings with a wealth of food and medical products. By the end of 2004, the global transgenic planting area had reached 81 million hectares, an increase of 20% compared with 2003. The Chinese government has approved the safe production of several genetically modified soybean, corn, canola and cotton lines in the Chinese market, including the MON863 corn line. With the widespread cultivation of genetically modified plants, the safety of genetically modified foods has attracted public attenti...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 张大兵杨立桃潘爱虎梁婉琪许诵辞尹长松章可为
Owner SHANGHAI JIAO TONG UNIV
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