Method of extracting anthraquinone analog compound from traditional Chinese medicine cassia seed

A technology of cassia seeds and compounds, applied in quinone separation/purification, organic chemistry, etc., can solve problems such as difficult to obtain monomer compounds

Inactive Publication Date: 2006-02-15
SOUTH CHINA NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is difficult to completely separate the chemical components in cassia seed by using these methods, and it is more difficult to obtain monomeric compounds

Method used

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  • Method of extracting anthraquinone analog compound from traditional Chinese medicine cassia seed
  • Method of extracting anthraquinone analog compound from traditional Chinese medicine cassia seed
  • Method of extracting anthraquinone analog compound from traditional Chinese medicine cassia seed

Examples

Experimental program
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Effect test

Embodiment 1

[0039] figure 2 This is the step (1) of the present invention-the extraction procedure diagram of Cassia seed solvent fraction. The Cassia seed used in this example was purchased from a pharmacy and produced in Anhui. Take 500g of Cassia Seeds, grind them into powder, and pass through a 100-200 mesh sieve. Using the solvent fractional extraction method, the solvent (cyclohexane-chloroform-ethyl acetate-n-butanol-ethanol-water) in the Soxhlet extractor is refluxed for 5 times in the order of polarity from small to large, and then combined The extraction liquid of each sub-step is recovered and the solvent is recovered to obtain the corresponding extract of the sub-step. The volume of solvent used each time is about twice that of Cassia seed powder. Each reflux extraction time is 60min. When changing to another solvent for extraction, the residue must be evaporated to dryness. Animal experiment results show that Cassia seed powder and its ethyl acetate extract, n-butanol extract, e...

Embodiment 2

[0044] Others are the same as in Example 1, except that in step (1), the cassia seed is 1000g, each reflux extraction 4 times in the Soxhlet extractor, the solvent volume used each time is 2.5 times that of the cassia seed powder, and the extraction time is 40 minutes each time; In step (2), the water extract is dissolved in 5 times the volume of distilled water, and the water-soluble part is extracted with n-butanol 8 times, each extraction volume is 1 times the volume of the aqueous solution, each extraction time is 40 minutes, and the combined extraction Liquid, evaporate the solvent to obtain the mixture A. The mixture A is fully dissolved in 5 times of 95% ethanol, and the mixture C that is insoluble in 95% ethanol is separated; in step (3), the mixture C is fully dissolved in 4 times of distilled water, and the solution part flows through the macroporous adsorption resin column , The effective part adsorbed on the column is eluted with 95% ethanol and water in sequence until...

Embodiment 3

[0046] Others are the same as in Example 1, except that in step (1), 1500g of Cassia seeds are refluxed and extracted 6 times in a Soxhlet extractor, and the solvent volume used each time is 1.5 times that of Cassia seeds powder. The best time for each extraction is 80 minutes; in step (2), the water extract is dissolved in 7.5 times the volume of distilled water, and the water-soluble part is extracted with n-butanol 4 times, and the volume of each extraction is 1.5 times the volume of the aqueous solution. Each extraction time is 20 min, the extracts are combined, and the solvent is evaporated to dryness to obtain mixture A. The mixture A is fully dissolved with 8 times of 95% ethanol, and the mixture C that is insoluble in 95% ethanol is separated; in step (3), the mixture C is fully dissolved in 3 times of distilled water, and the solution part flows through the macroporous adsorption resin column , The effective part adsorbed on the column is eluted with 95% ethanol and water...

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Abstract

The invention relates to a process for extracting anthraquinone compounds from cassia seed which comprises, (1) portion extraction, (2) preparation or mixture non-soluble to 95% ethanol, (3) isolating and purifying the mixture with microporous resin columns, (4) isolating and purifying the ethanol eluate and anthraquinone compounds with silica gel columns, thus obtaining active antihyperglycemic ingredients including emodin-1-O-beta-gentiobioside, chrysophanol-1-O-beta-gentiobioside, physcion-8-O-beta-gentiobioside, and chrysophanol-1-O-beta-D-glucopyranosyl-(1-3)-beta-D-glucopyranosyl-(1-6)-beta-D-glucopyranoside.

Description

Technical field [0001] The invention relates to a method for extracting anthraquinone compounds from Cassia obtusifolia L., a traditional Chinese medicine. That is, emodin-1-O-β-gentiobioside, chrysophanol-1-O-β-gentiobioside, and emodin methyl ether-8-O-β-gentiobioside are extracted from the traditional Chinese medicine Cassia seed. Glycosides and chrysophanol-1-O-β-D-glucopyranosyl-(1→3)-β-D-glucopyranosyl-(1→6)-β-D-glucopyranoside Anthraquinone compounds. Background technique [0002] Cassia seeds are the dried mature seeds of the legume Cassia obtusifolia L. or Cassia tora L., and are commonly used clinical Chinese medicines. A large number of studies have shown that: Cassia seed water decoction, ethanol, n-tincture alcohol, ethyl acetate and other organic solvent extracts, and Cassia seed extract have obvious lipid-lowering effects. Further research suggests that the main components of Cassia seed for lowering lipids are: anthraquinones, Cassia glycoside B, protein and so on...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C50/34C07C46/10
Inventor 李楚华李续娥郭宝江
Owner SOUTH CHINA NORMAL UNIVERSITY
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