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Molecule marking method for foreshowing and identifying chicken abdomen fat content by IGFBP2 gene

A molecular marker, chicken abdominal fat technology, applied in the field of animal molecular genetics, can solve the problem of unclear mechanism of action, affecting fat deposition, etc., to achieve the effect of low cost, simple operation, and speeding up the breeding process

Inactive Publication Date: 2006-05-10
NORTHEAST AGRICULTURAL UNIVERSITY
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Problems solved by technology

At the same time, it was found that the plasma content of IGFBP2 was negatively correlated with the plasma content of leptin (P<0.05). Leptin is an important regulator of fat metabolism and affects fat deposition. It can be seen that IGFBP2 has an impact on fat metabolism, but the relationship between IGFBP2 and Leptin The exact mechanism of action is unclear

Method used

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  • Molecule marking method for foreshowing and identifying chicken abdomen fat content by IGFBP2 gene

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Effect test

Embodiment 1

[0109] Embodiment 1, in different breed genotype and gene frequency statistical result

[0110] Utilize primers (IGFBP2F and IGFB2R) of the present invention to AA broiler, Hailan laying hens and local breeds Beijing oil chicken, white-eared chicken, Shi Qiza, a total of 5 random populations of individual genomic DNA were PCR amplified, and then SSCP analyze. A total of 3 genotypes were detected, which were named AA, AB, and BB genotypes (Fig. 1).

[0111] The genotype frequency and gene frequency of the gene in different chicken breeds were calculated and chi-square test was performed. The statistical results of genotype and gene frequency are shown in Table 1. Independence test (X 2 ) The overall results showed that the genotype frequency differences among varieties were extremely significant (P<0.01). A further analysis found that the difference between any other two chicken breeds reached a significant level (P<0.05). The A gene frequency and AA genotype frequency of ...

Embodiment 2

[0114] Example 2. Correlation between the polymorphism of chicken IGFBP2 gene and the abdominal fat traits of the sixth generation of broiler chicken high and low fat bidirectional selection lines

[0115] Utilize the primers (IGFBP2F and IGFB2R) of the present invention to perform PCR amplification on the genomic DNA of the sixth generation of broiler high-low-fat bidirectional selection line, and then perform SSCP analysis. A total of 3 genotypes were detected, which were named AA, AB, and BB genotypes (Fig. 1).

[0116] The least squares analysis was carried out on the three genotypes of IGFBP2 gene C1196A locus and the abdominal fat weight and abdominal fat rate of 227 individuals of the sixth generation. Significant effect (P<0.05) (Table 2).

[0117] Abdominal Fat Weight

[0118] Multiple comparisons of the least square means of the abdominal fat traits of the sixth generation chickens among the three genotypes showed that the abdominal fat weight and abdomi...

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Abstract

This invention provides a molecule marking method of indicating and identifying the fat quantum of the chicken abdomen with the IGFBP2 gene, which includes the following steps: designing a pair of inciting substances IGFBP2F and IGFBP2R according to the IGFBP2 gene list of chicken; making use of the inciting substances to amplify the gene groups DNA of the chick, then applying the SSCP method, doing the electrophoresis separation to the PCR production with the polypropene amide gelatin to check out one single alkali radicle differentiation location in the IGFBP2 gene3 control area; nominating the two pure type and mixing type in this location of the IGFBP2 gene of the chicken separately. This invention can accelerate the breeding process of the low fat meat chiken.

Description

[0001] (1) Fields [0002] The invention belongs to the field of animal molecular genetics, in particular to a method for detecting chicken body fat traits with molecular markers. (two), background technology [0003] After years of scientific breeding of broilers, the production performance of modern broilers has been significantly improved. However, while the early growth rate of broilers was significantly improved, the prominent problem that accompanied it was excessive abdominal fat deposition. Excessive fat deposition in broilers has many disadvantages: (1) Feed conversion efficiency is significantly reduced, because the deposition unit Adipose tissue by weight consumes three times more energy than deposited lean meat per unit weight; (2) reduces the ratio of carcass lean to adipose tissue, thereby reducing meat cut yield; (3) processors and consumers reduce broiler A large part of these fats deposited in the body (abdominal fat pad, fat around the gizzard, crop fat and ...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 李辉李志辉
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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