Method for inspecting quality of sensor chip, sample evaluating method, DNA chip, and protein chip
A sensor chip and protein technology, applied in the direction of material inspection products, biochemical equipment and methods, instruments, etc., can solve the problem of inability to distinguish the state or condition quality and performance of problem samples
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Embodiment 1
[0071] In Example 1, expression peaks of circadian rhythm control genes in individual circadian peripheral tissues at clock-time intervals were examined. The process is as follows.
[0072] Initially, the circadian rhythms of the mice used in the experiments were synchronized. More specifically, the mice were bred for 2 weeks in a room in which light conditions were maintained from 8 to 20 o'clock and dark conditions were maintained from 20 to 8 o'clock the next day, so that the circadian rhythms of the mice were synchronized. Note that for the experiments, ICR mice (male, 5-week-old) purchased from Nihon SLC Abushikikaisha were used.
[0073] Next, the heart, lung, liver, stomach, spleen, and testis of the mice were sampled as circadian peripheral tissues at given time intervals, respectively, and immediately frozen with liquid nitrogen. Note that the sampling time for each internal organ is set to be every four hours at 8 o'clock determined immediately after synchronizatio...
Embodiment 2
[0083] In Example 2, the expression level of Per1 in samples collected and prepared from lungs was compared with the aforementioned data (see FIG. 1 ) for quality check and sample evaluation.
[0084] Initially, as in Example 1, the circadian rhythms of the mice used in this experiment were synchronized. Thereafter, lungs of each mouse were sampled at given times, followed by immediate freezing with liquid nitrogen. Sampling time points were set at every four hours (CT0, CT4, CT8, CT12, CT16, CT20) immediately after (CT0) synchronization of the circadian rhythm. Note that the label CT refers to the circadian rhythm time in which the lighting condition-start time is set at 0 o'clock (CT0).
[0085] Next, each lung (ling) sample was homogenized, after which total RNA was extracted from each organ using the Promega Total SV RNAIsolation Kit (Promega Inc.). Next, quantitative real-time RT-PCR (quantitative real-time reverse transcription polymerase reaction) was performed to mea...
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